Genomes of Three Methylotrophs from a Single Niche Reveal the Genetic and Metabolic Divergence of the Methylophilaceae

被引：46

作者：

Lapidus, Alla ^{[2
]}

Clum, Alicia ^{[2
]}

LaButti, Kurt ^{[2
]}

Kaluzhnaya, Marina G. ^{[3
]}

Lim, Sujung ^{[3
]}

Beck, David A. C. ^{[1
,4
]}

del Rio, Tijana Glavina ^{[2
]}

Nolan, Matt ^{[2
]}

Mavromatis, Konstantinos ^{[2
]}

Huntemann, Marcel ^{[2
]}

Lucas, Susan ^{[2
]}

Lidstrom, Mary E. ^{[1
,3
]}

Ivanova, Natalia ^{[2
]}

Chistoserdova, Ludmila ^{[1
]}

机构：

[1] Univ Washington, Dept Chem Engn, Seattle, WA 98195 USA

[2] DOE Joint Genome Inst, Walnut Creek, CA USA

[3] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA

[4] Univ Washington, eSci Inst, Seattle, WA 98195 USA

来源：

JOURNAL OF BACTERIOLOGY | 2011年 / 193卷 / 15期

基金：

美国国家科学基金会;

关键词：

METHYLOBACTERIUM-EXTORQUENS AM1; RIBULOSE MONOPHOSPHATE CYCLE; METHYLOBACILLUS-FLAGELLATUS; METHANOL DEHYDROGENASE; IDENTIFICATION; OXIDATION; SEDIMENT; ENZYME; LAKE; RNA;

D O I：

10.1128/JB.00404-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The genomes of three representatives of the family Methylophilaceae, Methylotenera mobilis JLW8, Methylotenera versatilis 301, and Methylovorus glucosetrophus SIP3-4, all isolated from a single study site, Lake Washington in Seattle, WA, were completely sequenced. These were compared to each other and to the previously published genomes of Methylobacillus flagellatus KT and an unclassified Methylophilales strain, HTCC2181. Comparative analysis revealed that the core genome of Methylophilaceae may be as small as approximately 600 genes, while the pangenome may be as large as approximately 6,000 genes. Significant divergence between the genomes in terms of both gene content and gene and protein conservation was uncovered, including the varied presence of certain genes involved in methylotrophy. Overall, our data demonstrate that metabolic potentials can vary significantly between different species of Methylophilaceae, including organisms inhabiting the very same environment. These data suggest that genetic divergence among the members of this family may be responsible for their specialized and nonredundant functions in C-1 cycling, which in turn suggests means for their successful coexistence in their specific ecological niches.

引用

页码：3757 / 3764

页数：8

共 39 条

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