Overexpression of human selenoprotein M differentially regulates the concentrations of antioxidants and H2O2, the activity of antioxidant enzymes, and the composition of white blood cells in a transgenic rat

被引：1

作者：

Hwang, Dae Youn ^{[1
]}

Sin, Ji Soon ^{[1
]}

Kim, Min Sun ^{[1
]}

Yim, Su Youn ^{[1
]}

Kim, Yong Kyu ^{[1
]}

Kim, Chuel Kyu ^{[1
]}

Kim, Byoung Guk ^{[1
]}

Shim, Sun Bo ^{[1
]}

Jee, Seung Wan ^{[1
]}

Lee, Su Hae ^{[1
]}

Bae, Chang Joon ^{[1
]}

Lee, Byoung Chun ^{[1
]}

Jang, Mee Kyung ^{[1
]}

Cho, Jung Sik ^{[1
]}

Chae, Kab Ryong ^{[1
]}

机构：

[1] Korean FDA, Natl Inst Toxicol Res, Lab Anim Resources Team, Seoul 122704, South Korea

来源：

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2008年 / 21卷 / 02期

关键词：

selenoprotein M; transgenic rat; 2,2 '-azobiz(2-amidinopropane) dihydrochloride; superoxide dismutase; glutathione peroxidase;

D O I：

暂无

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Selenoprotein is associated with a variety of serious diseases, including infectious diseases, neurodegenerative disorders, cancer and cardiovascular disease. The aim of this study was to produce a new transgenic (Tg) rat expressing human selenoprotein M (SelM) in order to examine the protective function of the antioxidant status in vivo. To achieve this, a new lineage of Tg rats was produced by the micro-injection of pCMV/GFP-hSelM constructs into a fertilized rat egg. Several conclusions can be drawn based on the results of the present study. The human SelM gene was successfully expressed at both the transcription and protein levels in the CMV/GFP-hSelM Tg rats. This Tg rat showed a different enzyme activity for the antioxidant protein in the various tissues examined. In response to the 2,2'-azobiz(2-amidinopropane) dihydrochloride (AAPH) injection, the Tg rats showed a lower level of antioxidant and H2O2 concentration as the activity of the antioxidant enzyme was maintained at a higher level in the Tg rats than in the non-Tg rats. Also, the neutrophil-to-lymphocyte ratio was significantly increased in this Tg rat, even though the level of corticosterone remained unchanged in both genotypes. Thus, the results of this study demonstrated that the CMV/GFP-hSelM Tg rat can serve as an animal model for the maintenance of a high level of antioxidant status and can be used to study the biological function of selenoprotein in infectious diseases, cardiovascular disease and cancer.

引用

页码：169 / 179

页数：11

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