PTRF-Cavin, a conserved cytoplasmic protein required for Caveola formation and function

被引：566

作者：

Hill, Michelle M. ^{[1
,2
]}

Bastiani, Michele ^{[1
,2
]}

Luetterforst, Robert ^{[1
,2
]}

Kirkham, Matthew ^{[1
,2
]}

Kirkham, Annika ^{[1
,2
]}

Nixon, Susan J. ^{[1
,2
]}

Walser, Piers ^{[1
,2
]}

Abankwa, Daniel ^{[1
]}

Oorschot, Viola M. J. ^{[1
,2
]}

Martin, Sally ^{[1
,2
]}

Hancock, John F. ^{[1
]}

Parton, Robert G. ^{[1
,2
]}

机构：

[1] Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia

[2] Univ Queensland, Ctr Microscopy & Microanal, Brisbane, Qld 4072, Australia

来源：

CELL | 2008年 / 132卷 / 01期

关键词：

D O I：

10.1016/j.cell.2007.11.042

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Caveolae are abundant cell-surface organelles involved in lipid regulation and endocytosis. We used comparative proteomics to identify PTRF ( also called Cav-p60, Cavin) as a putative caveolar coat protein. PTRF-Cavin selectively associates with mature caveolae at the plasma membrane but not Golgi-localized caveolin. In prostate cancer PC3 cells, and during development of zebrafish notochord, lack of PTRF-Cavin expression correlates with lack of caveolae, and caveolin resides on flat plasma membrane. Expression of PTRF-Cavin in PC3 cells is sufficient to cause formation of caveolae. Knockdown of PTRF-Cavin reduces caveolae density, both in mammalian cells and in the zebrafish. Caveolin remains on the plasma membrane in PTRF-Cavin knockdown cells but exhibits increased lateral mobility and accelerated lysosomal degradation. We conclude that PTRF-Cavin is required for caveola formation and sequestration of mobile caveolin into immobile caveolae.

引用

页码：113 / 124

页数：12

共 38 条

[1] Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes [J].