As one of the key techniques of vision coordinates measurement, camera internal parameters calibration is regarded by technologists and has been researched a lot. Based on the study of regular internal parameters calibration methods such as plane calibration, two-plane calibration, vanishing points calibration, stereo calibration and so on, combined with the requirement of large space vision coordinates measurement for camera internal parameters calibration, this paper brings forward independent internal parameters calibration and virtual stereo calibration methods. A special calibration equipment is used in independent internal parameters calibration to calibrate original points coordinates of camera and distortion parameters of lens, and a virtual target board and bundle adjustment algorithm are used in virtual stereo calibration to calibrate internal parameters. They can be used in large space vision coordinates measurement and get high calibration accuracy.