Prevention of doxorubicin sorptive losses in drug delivery studies using polyethylene glycol

被引：10

作者：

Curry, Dennis ^{[1
,2
,3
]}

Scheller, Hope ^{[1
,2
]}

Lu, Mingsheng ^{[1
]}

Mkandawire, Martin ^{[1
,4
]}

Servos, Mark R. ^{[3
]}

Cui, Shufen ^{[5
]}

Zhang, Xu ^{[1
,3
,4
]}

Oakes, Ken D. ^{[1
,2
]}

机构：

[1] Cape Breton Univ, Verschuren Ctr Sustainabil Energy & Environm, Sydney, NS B1P 6L2, Canada

[2] Cape Breton Univ, Dept Biol, Sydney, NS B1P 6L2, Canada

[3] Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada

[4] Cape Breton Univ, Dept Chem, Sydney, NS B1P 6L2, Canada

[5] Shenzhen Polytech, Dept Biol Appl Engn, Shenzhen Key Lab Fermentat Purificat & Anal, Shenzhen 518055, Peoples R China

来源：

RSC ADVANCES | 2015年 / 5卷 / 33期

基金：

加拿大健康研究院;

关键词：

BLOCK-COPOLYMER MICELLES; GOLD NANOPARTICLES; ADSORPTION; AGENT; ENCAPSULATION; DEGRADATION; CONJUGATE; STABILITY; CARCINOMA; PH;

D O I：

10.1039/c5ra01799h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The nonspecific sorption of hydrophobic pharmaceuticals on reaction vessel surfaces raises serious analytical challenges for their accurate quantification. Systematic error due to sorptive loss of analytes may result in significant overestimation of drug loading on nanomaterial-based Drug Delivery Systems (DDS), leading to inaccurate determinations of dosage and DDS efficiency. We evaluated sorptive losses of doxorubicin (DOX), an effective chemotherapeutic, in polystyrene based 96-well plates, and proposed a simple but effective method to prevent the nonspecific sorption of DOX using trace concentrations of polyethylene glycol (PEG). Relative to widely used proteinaceous and surfactant surface blocking agents, PEG is effective, easy to use, and does not interfere with drug loading to the DDS.

引用

页码：25693 / 25698

页数：6

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