Rupturing Giant Plasma Membrane Vesicles to Form Micron-sized Supported Cell Plasma Membranes with Native Transmembrane Proteins

被引：21

作者：

Chiang, Po-Chieh ^{[1
]}

Tanady, Kevin ^{[1
]}

Huang, Ling-Ting ^{[1
]}

Chao, Ling ^{[1
]}

机构：

[1] Natl Taiwan Univ, Dept Chem Engn, Taipei, Taiwan

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

LIPID-BILAYER FORMATION; DIFFUSION; PROTEOLIPOSOMES; MOBILITY; EVENTS; GLASS;

D O I：

10.1038/s41598-017-15103-3

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Being able to directly obtain micron-sized cell blebs, giant plasma membrane vesicles (GPMVs), with native membrane proteins and deposit them on a planar support to form supported plasma membranes could allow the membrane proteins to be studied by various surface analytical tools in native-like bilayer environments. However, GPMVs do not easily rupture on conventional supports because of their high protein and cholesterol contents. Here, we demonstrate the possibility of using compression generated by the air-water interface to efficiently rupture GPMVs to form micron-sized supported membranes with native plasma membrane proteins. We demonstrated that not only lipid but also a native transmembrane protein in HeLa cells, Aquaporin 3 (AQP3), is mobile in the supported membrane platform. This convenient method for generating micron-sized supported membrane patches with mobile native transmembrane proteins could not only facilitate the study of membrane proteins by surface analytical tools, but could also enable us to use native membrane proteins for bio-sensing applications.

引用

页数：8

共 42 条

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