The Microbial Communities in Male First Catch Urine Are Highly Similar to Those in Paired Urethral Swab Specimens

被引：150

作者：

Dong, Qunfeng ^{[1
,2
]}

Nelson, David E. ^{[3
]}

Toh, Evelyn ^{[3
]}

Diao, Lixia ^{[4
]}

Gao, Xiang ^{[1
]}

Fortenberry, J. Dennis ^{[5
]}

Van Der Pol, Barbara ^{[6
]}

机构：

[1] Univ N Texas, Dept Biol, Denton, TX 76203 USA

[2] Univ N Texas, Dept Comp Sci & Engn, Denton, TX 76203 USA

[3] Indiana Univ, Dept Biol, Bloomington, IN USA

[4] Univ Texas MD Anderson Canc Ctr, Dept Bioinformat & Computat Biol, Houston, TX 77030 USA

[5] Indiana Univ Sch Med, Dept Pediat, Sect Adolescent Med, Indianapolis, IN USA

[6] Indiana Univ, Sch Publ Hlth, Bloomington, IN USA

来源：

PLOS ONE | 2011年 / 6卷 / 05期

基金：

美国国家卫生研究院;

关键词：

NONGONOCOCCAL URETHRITIS; ASSOCIATION; INFECTION; CHLAMYDIA; VAGINALIS;

D O I：

10.1371/journal.pone.0019709

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Urine is the CDC-recommended specimen for STI testing. It was unknown if the bacterial communities (microbiomes) in urine reflected those in the distal male urethra. We compared microbiomes of 32 paired urine and urethral swab specimens obtained from adult men attending an STD clinic, by 16S rRNA PCR and deep pyrosequencing. Microbiomes of urine and swabs were remarkably similar, regardless of STI status of the subjects. Thus, urine can be used to characterize urethral microbiomes when swabs are undesirable, such as in population-based studies of the urethral microbiome or where multiple sampling of participants is required.

引用

页数：5

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