Probing molecular interactions in single and live cells with fluorescence spectroscopy

被引：5
|
作者：
Kumar, U
Baragli, A
Patel, RC [1 ]
机构：
[1] Clarkson Univ, Dept Chem & Phys, Potsdam, NY 13699 USA
[2] Neural Connect, Potsdam, NY 13676 USA
[3] McGill Univ, Hugh Fraser Neurosci Labs, Dept Med, Montreal, PQ H3A 1A1, Canada
[4] McGill Univ, Hugh Fraser Neurosci Labs, Dept Pharmacol & Therapeut, Montreal, PQ H3A 1A1, Canada
[5] Royal Victoria Hosp, Montreal, PQ H3A 1A1, Canada
来源：
TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2003年 / 22卷 / 09期
关键词：
fluorescence spectroscopy; G-protein-coupled-receptors; live cells;
D O I：
10.1016/S0165-9936(03)00601-0
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
G-protein-coupled receptors (GPCRs) are the largest class of cell-surface receptors and are encoded by over 1000 genes in the human genome. Their activation mechanism is investigated using live cells, cultured cortical neurons, and brain slices in combination with fluorescence resonance energy transfer (FRET) and fluorescence correlation spectroscopy (FCS) including auto and cross correlation. (C) 2003 Published by Elsevier B.V.
引用
收藏
页码：537 / 543
页数：7
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