Aqueous Two-Phase Extraction Combined with Chromatography: New Strategies for Preparative Separation and Purification of Capsaicin from Capsicum Oleoresin

被引:10
作者
Zhao, Pei-pei [1 ]
Lu, Yan-min [1 ]
Tan, Cong-ping [1 ]
Liang, Yan [1 ]
Cui, Bo [1 ]
机构
[1] Qilu Univ Technol, Coll Food & Bioengn, Shandong Prov Key Lab Fine Chem, Jinan 250353, Shandong, Peoples R China
关键词
Capsaicin; Separation; Aqueous two-phase extraction; Purification; Chromatography; Reverse-phase resin; PERFORMANCE LIQUID-CHROMATOGRAPHY; MACROPOROUS ADSORPTION RESINS; SYSTEMS; RECOVERY; DIHYDROCAPSAICIN; ADSORBENTS; FLAVONOIDS; PARTITION;
D O I
10.1007/s12010-014-1303-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Capsaicin was preparatively separated and purified from capsicum oleoresin with a new method combined with aqueous two-phase extraction (ATPE) and chromatography. Screening experiments of ATPE systems containing salts and hydrophilic alcohols showed that potassium carbonate/ethanol system was the most suitable system for capsaicin recovery among the systems considered. Response surface methodology was used to determine an optimized aqueous two-phase system for the extraction of capsaicin from capsaicin oleoresin. In a 20 % (w/w) ethanol/22.3 % (w/w) potassium carbonate system, 85.4 % of the capsaicin was recovered in the top ethanol-rich phase while most oil and capsanthin ester were removed in the interphase. The capsaicinoid extract was then subjected to two chromatographic steps using D101 macroporous resin and inexpensive SKP-10-4300 reverse-phase resin first applied for the purification of capsaicin. After simple optimization of loading/elution conditions for D101 macroporous resin chromatography and SKP-10-4300 reverse-phase resin chromatography, the purities of capsaicin were improved from 7 to 85 %. In the two chromatography processes, the recoveries of capsaicin were 93 and 80 % respectively; the productivities of capsaicin were 1.86 and 4.2 (g capsaicin/L resin) per day respectively. It is worth mentioning that a by-product of capsaicin production was also obtained with a high purity (90 %).
引用
收藏
页码:1018 / 1034
页数:17
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