共 50 条
Aqueous Two-Phase Extraction Combined with Chromatography: New Strategies for Preparative Separation and Purification of Capsaicin from Capsicum Oleoresin
被引:10
作者:
Zhao, Pei-pei
[1
]
Lu, Yan-min
[1
]
Tan, Cong-ping
[1
]
Liang, Yan
[1
]
Cui, Bo
[1
]
机构:
[1] Qilu Univ Technol, Coll Food & Bioengn, Shandong Prov Key Lab Fine Chem, Jinan 250353, Shandong, Peoples R China
关键词:
Capsaicin;
Separation;
Aqueous two-phase extraction;
Purification;
Chromatography;
Reverse-phase resin;
PERFORMANCE LIQUID-CHROMATOGRAPHY;
MACROPOROUS ADSORPTION RESINS;
SYSTEMS;
RECOVERY;
DIHYDROCAPSAICIN;
ADSORBENTS;
FLAVONOIDS;
PARTITION;
D O I:
10.1007/s12010-014-1303-6
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Capsaicin was preparatively separated and purified from capsicum oleoresin with a new method combined with aqueous two-phase extraction (ATPE) and chromatography. Screening experiments of ATPE systems containing salts and hydrophilic alcohols showed that potassium carbonate/ethanol system was the most suitable system for capsaicin recovery among the systems considered. Response surface methodology was used to determine an optimized aqueous two-phase system for the extraction of capsaicin from capsaicin oleoresin. In a 20 % (w/w) ethanol/22.3 % (w/w) potassium carbonate system, 85.4 % of the capsaicin was recovered in the top ethanol-rich phase while most oil and capsanthin ester were removed in the interphase. The capsaicinoid extract was then subjected to two chromatographic steps using D101 macroporous resin and inexpensive SKP-10-4300 reverse-phase resin first applied for the purification of capsaicin. After simple optimization of loading/elution conditions for D101 macroporous resin chromatography and SKP-10-4300 reverse-phase resin chromatography, the purities of capsaicin were improved from 7 to 85 %. In the two chromatography processes, the recoveries of capsaicin were 93 and 80 % respectively; the productivities of capsaicin were 1.86 and 4.2 (g capsaicin/L resin) per day respectively. It is worth mentioning that a by-product of capsaicin production was also obtained with a high purity (90 %).
引用
收藏
页码:1018 / 1034
页数:17
相关论文
共 50 条