Quantitative assessment of spinal cord injury using circularly polarized coherent anti-Stokes Raman scattering microscopy

被引：9

作者：

Bae, Kideog ^{[1
]}

Zheng, Wei ^{[1
,2
]}

Huang, Zhiwei ^{[1
]}

机构：

[1] Natl Univ Singapore, Dept Biomed Engn, Opt Bioimaging Lab, Fac Engn, Singapore 117576, Singapore

[2] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Med, Singapore 119260, Singapore

来源：

APPLIED PHYSICS LETTERS | 2017年 / 111卷 / 06期

基金：

英国医学研究理事会;

关键词：

MYELIN;

D O I：

10.1063/1.4991792

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We report the quantitative assessment of spinal cord injury using the circularly polarized coherent anti-Stokes Raman scattering (CP-CARS) technique together with Stokes parameters in the Poincare sphere. The pump and Stokes excitation beams are circularly polarized to suppress both the linear polarization-dependent artifacts and the nonresonant background of tissue CARS imaging, enabling quantitative CP-CARS image analysis. This study shows that CP-CARS imaging uncovers significantly increased phase retardance of injured spinal cord tissue as compared to normal tissue, suggesting that CP-CARS is an appealing label-free imaging tool for determining the degree of tissue phase retardance, which could serve as a unique diagnostic parameter associated with nervous tissue injury. Published by AIP Publishing.

引用

页数：4

共 17 条

[1] In vivo evaluation of demyelination and remyelination in a nerve crush injury model [J].

Belanger, E. ;

Henry, F. P. ;

Vallee, R. ;

Randolph, M. A. ;

Kochevar, I. E. ;

Winograd, J. M. ;

Lin, C. P. ;

Cote, D. .

BIOMEDICAL OPTICS EXPRESS, 2011, 2 (09) :2698-2708

[2] Quantitative myelin imaging with coherent anti-Stokes Raman scattering microscopy: alleviating the excitation polarization dependence with circularly polarized laser beams [J].

Belanger, E. ;

Begin, S. ;

Laffray, S. ;

De Koninck, Y. ;

Vallee, R. ;

Cote, D. .

OPTICS EXPRESS, 2009, 17 (21) :18419-18432

[3]

Born M., 1999, Principles of optics, Vseventh

[4] MEASUREMENT OF THE 4 STOKES POLARIZATION PARAMETERS WITH A SINGLE CIRCULAR POLARIZER [J].

COLLETT, E .

OPTICS COMMUNICATIONS, 1984, 52 (02) :77-80

[5] Understanding fiber mixture by simulation in 3D Polarized Light Imaging [J].

Dohmen, Melanie ;

Menzel, Miriam ;

Wiese, Hendrik ;

Reckfort, Julia ;

Hanke, Frederike ;

Pietrzyk, Uwe ;

Zilles, Karl ;

Amunts, Katrin ;

Axer, Markus .

NEUROIMAGE, 2015, 111 :464-475

[6] Promoting glutathione synthesis after spinal cord trauma decreases secondary damage and promotes retention of function [J].

Kamencic, H ;

Griebel, RW ;

Lyon, AW ;

Paterson, PG ;

Juurlink, BHJ .

FASEB JOURNAL, 2001, 15 (01) :243-250

[7] Annular aperture-detected coherent anti-Stokes Raman scattering microscopy for high contrast vibrational imaging [J].

Lin, Jian ;

Lu, Fake ;

Zheng, Wei ;

Huang, Zhiwei .

APPLIED PHYSICS LETTERS, 2010, 97 (08)

[8] Interferometric polarization coherent anti-Stokes Raman scattering (IP-CARS) microscopy [J].

Lu, Fake ;

Zheng, Wei ;

Sheppard, Colin ;

Huang, Zhiwei .

OPTICS LETTERS, 2008, 33 (06) :602-604

[9] Elliptically polarized coherent anti-Stokes Raman scattering microscopy [J].

Lu, Fake ;

Zheng, Wei ;

Huang, Zhiwei .

OPTICS LETTERS, 2008, 33 (23) :2842-2844

[10] Principles of diffusion tensor imaging and its applications to basic neuroscience research [J].

Mori, Susumu ;

Zhang, Jiangyang .

NEURON, 2006, 51 (05) :527-539

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