Paraburkholderia nodosa is the main N2-fixing species trapped by promiscuous common bean (Phaseolus vulgaris L.) in the Brazilian 'Cerradao'

被引：38

作者：

Dall'Agnol, Rebeca F. ^{[1
,2
]}

Plotegher, Fabio ^{[1
]}

Souza, Renata C. ^{[1
,3
]}

Mendes, Ieda C. ^{[4
]}

dos Reis Junior, Fabio B. ^{[4
]}

Bena, Gilles ^{[5
]}

Moulin, Lionel ^{[5
]}

Hungria, Mariangela ^{[1
,3
]}

机构：

[1] Embrapa Soja, Soil Biotechnol Lab, CP 231, BR-86001970 Londrina, PR, Brazil

[2] Univ Estadual Londrina, Dept Biochem & Biotechnol, CP 10011, BR-86057970 Londrina, PR, Brazil

[3] Univ Fed Parana, Dept Microbiol, CP 19031, BR-81531990 Curitiba, PR, Brazil

[4] Embrapa Cerrados, Dept Soil Microbiol, CP 08223, BR-73301970 Planaltina, DF, Brazil

[5] Univ Montpellier, Cirad, IRD, IPME, F-34394 Montpellier, France

来源：

FEMS MICROBIOLOGY ECOLOGY | 2016年 / 92卷 / 08期

关键词：

beta-rhizobia; phylogeny of prokaryotes; biological N-2 fixation; Cerrado; SP-NOV; GENETIC DIVERSITY; BURKHOLDERIA-PHYMATUM; NITROGEN-FIXATION; MIMOSA SPP; RHIZOBIAL SYMBIONTS; ROOT-NODULES; FIXING N-2; STRAINS; LEGUMES;

D O I：

10.1093/femsec/fiw108

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The bacterial genus Burkholderia comprises species occupying several habitats, including a group of symbionts of leguminous plants-also called beta-rhizobia-that has been recently ascribed to the new genus Paraburkholderia. We used common bean (Phaseolus vulgaris L.) plants to trap rhizobia from an undisturbed soil of the Brazilian Cerrado under the vegetation type 'Cerradao'. Genetic characterization started with the analyses of 181 isolates by BOX-PCR, where the majority revealed unique profiles, indicating high inter- and intra-species diversity. Restriction fragment length polymorphism-PCR of the 16S rRNA of representative strains of the BOX-PCR groups indicated two main clusters, and gene-sequencing analysis identified the minority (27%) as Rhizobium and the majority (73%) as Paraburkholderia. Phylogenetic analyses of the 16S rRNA and housekeeping (recA and gyrB) genes positioned all strains of the second cluster in the species P. nodosa, and the phylogeny of a symbiotic gene-nodC-was in agreement with the conserved genes. All isolates were stable vis-A -vis nodulating common bean, but, in general, with a low capacity for fixing N-2, although some effective strains were identified. The predominance of P. nodosa might be associated with the edaphic properties of the Cerrado biome, and might represent an important role in terms of maintenance of the ecosystem, which is characterized by acid soils with high saturation of aluminum and low N-2 content.Study of the diversity of nitrogen-fixing symbionts from a 'hot spot area', the Brazilian 'Cerradao', reveals that Paraburkholderia nodosa is the main symbiont trapped by promiscuous common bean (Phaseolus vulgaris L.).Study of the diversity of nitrogen-fixing symbionts from a 'hot spot area', the Brazilian 'Cerradao', reveals that Paraburkholderia nodosa is the main symbiont trapped by promiscuous common bean (Phaseolus vulgaris L.).

引用

页数：14

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