The Thermus thermophilus 5S rRNA-protein complex:: Identification of specific binding sites for proteins L5 and L18 in the 5S rRNA

被引：8

作者：

Gongadze, GM ^{[1
]}

Perederina, AA ^{[1
]}

Meshcheryakov, VA ^{[1
]}

Fedorov, RV ^{[1
]}

Moskalenko, SE ^{[1
]}

Rak, AV ^{[1
]}

Serganov, AA ^{[1
]}

Shcherbakov, DV ^{[1
]}

Nikonov, SV ^{[1
]}

Garber, MB ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Prot Res, Pushchino 142290, Moscow Region, Russia

来源：

MOLECULAR BIOLOGY | 2001年 / 35卷 / 04期

基金：

俄罗斯基础研究基金会;

关键词：

Thermus thermophilus; bacterial ribosome; 5S rRNA; ribosomal proteins; RNA-protein interactions;

D O I：

10.1023/A:1010562707875

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three 5S rRNA-binding ribosomal proteins (L5, L18, TL5) of extremely thermophilic bacterium Thermus thermophilus have earlier been isolated. Structural analysis of their complexes with rRNA requires identification of their binding sites in the 5S rRNA. Previously, a TL5-binding site has been identified, a TL5-RNA complex crystallized, and its structure determined to 2.3 Angstrom. The sites for L5 and L18 were characterized, and two corresponding 5S rRNA fragments constructed. Of these, a 34-nt fragment specifically interacted with L5, and a 55-nt fragment interacted with L5, L18, and with both proteins. The 34-nt fragment-L5 complex was crystallized; the crystals are suitable for high-resolution X-ray analysis.

引用

页码：521 / 526

页数：6

共 32 条

[1] Another piece of the ribosome:: solution structure of S16 and its location in the 30S subunit
Allard, P
Rak, AV
Wimberly, BT
Clemons, WM
Kalinin, A
Helgstrand, M
Garber, MB
Ramakrishnan, V
Härd, T
[J]. STRUCTURE WITH FOLDING & DESIGN, 2000, 8 (08): : 875 - 882
[2] The complete atomic structure of the large ribosomal subunit at 2.4 Å resolution
Ban, N
Nissen, P
Hansen, J
Moore, PB
Steitz, TA
[J]. SCIENCE, 2000, 289 (5481) : 905 - 920
[3] X-ray crystal structures of 70S ribosome functional complexes
Cate, JH
Yusupov, MM
Yusupova, GZ
Earnest, TN
Noller, HF
[J]. SCIENCE, 1999, 285 (5436) : 2095 - 2104
[4] SECONDARY STRUCTURE OF PROKARYOTIC 5-S RIBOSOMAL RIBONUCLEIC-ACIDS - A STUDY WITH RIBONUCLEASES
DOUTHWAITE, S
GARRETT, RA
[J]. BIOCHEMISTRY, 1981, 20 (25) : 7301 - 7307
[5] RIBONUCLEASE-RESISTANT REGION OF 5S-RNA AND ITS RELATION TO THE RNA-BINDING SITES OF PROTEINS L18 AND L25
DOUTHWAITE, S
GARRETT, RA
WAGNER, R
FEUNTEUN, J
[J]. NUCLEIC ACIDS RESEARCH, 1979, 6 (07) : 2453 - 2470
[6] FEDOROV R, IN PRESS ACTA CRYSTA
[7] 5S rRNA binding ribosomal proteins from Thermus thermophilus: Identification and some structural properties
Gongadze, G
Kashparov, I
Lorenz, S
Schroeder, W
Erdmann, VA
Liljas, A
Garber, M
[J]. FEBS LETTERS, 1996, 386 (2-3) : 260 - 262
[8] RIBOSOMAL-PROTEINS, TL4 AND TL5, FROM THERMUS-THERMOPHILUS FORM HYBRID COMPLEXES WITH 5 S RIBOSOMAL-RNA FROM DIFFERENT MICROORGANISMS
GONGADZE, GM
TISHCHENKO, SV
SEDELNIKOVA, SE
GARBER, MB
[J]. FEBS LETTERS, 1993, 330 (01) : 46 - 48
[9] N-terminal domain, residues 1-91, of ribosomal protein TL5 from Thermus thermophilus binds specifically and strongly to the region of 5S rRNA containing loop E
Gongadze, GM
Meshcheryakov, VA
Serganov, AA
Fomenkova, NP
Mudrik, ES
Jonsson, BH
Liljas, A
Nikonov, SV
Garber, MB
[J]. FEBS LETTERS, 1999, 451 (01) : 51 - 55
[10] A ribosomal protein from Thermus thermophilus is homologous to a general shock protein
Gryaznova, OI
Davydova, NL
Gongadze, GM
Jonsson, BH
Garber, MB
Liljas, A
[J]. BIOCHIMIE, 1996, 78 (11-12) : 915 - 919

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