Combination of PAGE and LA-ICP-MS as an analytical workflow in metallomics: state of the art, new quantification strategies, advantages and limitations

被引：49

作者：

Sussulini, Alessandra ^{[1
]}

Becker, Johanna Sabine ^{[1
]}

机构：

[1] Forschungszentrum Julich, Cent Div Analyt Chem, D-52425 Julich, Germany

来源：

METALLOMICS | 2011年 / 3卷 / 12期

关键词：

PLASMA-MASS SPECTROMETRY; GEL-ELECTROPHORESIS; ANALYTICAL-CHEMISTRY; QUANTITATIVE PROTEOMICS; BINDING PROTEINS; SPECIATION; METALS; METALLOPROTEOMICS; IDENTIFICATION; SEPARATION;

D O I：

10.1039/c1mt00116g

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Metallomics (more specifically, metalloproteomics) is an emerging field that encompasses the role, uptake, transport and storage of trace metals, which are essential to preserve the functions of proteins within a biological system. The current strategies for metal-binding and metalloprotein analysis based on the combination of polyacrylamide gel electrophoresis (PAGE) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) are discussed in this review. The advantages, limitations and the most recently developed and applied quantification approaches for this methodology are also described.

引用

页码：1271 / 1279

页数：9

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