Nitration is exclusive to defense-related PR-1, PR-3 and PR-5 proteins in tobacco leaves

被引：11
|
作者：
Takahashi, Misa [1 ]
Shigeto, Jun [1 ]
Izumi, Shunsuke [1 ]
Yoshizato, Katsutoshi [2 ]
Morikawa, Hiromichi [1 ]
机构：
[1] Hiroshima Univ, Dept Math & Life Sci, Higashihiroshima, Japan
[2] Hiroshima Univ, Dept Biol Sci, Higashihiroshima, Hiroshima, Japan
来源：
PLANT SIGNALING & BEHAVIOR | 2016年 / 11卷 / 07期
基金：
日本学术振兴会;
关键词：
Apoplast; defense/immunity-related proteins; nitrogen dioxide; pathogenesis-related proteins; PR-1; PR-3; PR-5; protein tyrosine nitration; tobacco; vacuole; PATHOGENESIS-RELATED PROTEINS; ATMOSPHERIC NITROGEN-DIOXIDE; TYROSINE NITRATION; NITRIC-OXIDE; ARABIDOPSIS; REDUCTASE; INHIBITION; INDUCTION; TIME;
D O I：
10.1080/15592324.2016.1197464
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Protein tyrosine nitration is an important post-translational modification. A variety of nitrated proteins are reported in Arabidopsis leaves and seedlings, sunflower hypocotyls, and pea roots. The identities of nitrated proteins are species-/organ-specific, and chloroplast proteins are most nitratable in leaves. However, precise mechanism is unclear. Here, we investigated nitroproteome in tobacco leaves following exposure to nitrogen dioxide. Proteins were extracted, electrophoresed and immunoblotted using an anti-3-nitrotyrosine antibody. Mass spectrometry and FASTA search identified for the first time an exclusive nitration of pathogenesis-related proteins, PR-1, PR-3 and PR-5, which are reportedly located in the apoplast or the vacuole. Furthermore, Tyr36 of thaumatin-like protein E2 was identfied as a nitration site. The underlying mechanism and physiological relevance are discussed.
引用
收藏
页数：5
相关论文
共 37 条
  • [21] Genome-wide analysis of pathogenesis-related protein 1 (PR-1) gene family from Musa spp. and its role in defense response during stresses
    Anuradha, Chelliah
    Chandrasekar, Arumugam
    Backiyarani, Suthanthiram
    Thangavelu, Raman
    Giribabu, Palaniappan
    Uma, Subbaraya
    GENE, 2022, 821
  • [22] Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen
    Chow, LP
    Chiu, LL
    Khoo, KH
    Peng, HJ
    Yang, SY
    Huang, SW
    Su, SN
    FEBS JOURNAL, 2005, 272 (24) : 6218 - 6227
  • [23] Exploring common bean's ' s defense arsenal: Genome-wide characterization of PR-1 gene family and its transcriptional response to Colletotrichum lindemuthianum inoculation
    Trajano Mangueira de Melo, Ana Luiza
    Carneiro Leao, Mariele Porto
    da Silva, Manasses Daniel
    Santos, Cleidiane Macedo
    da Silva, Rahisa Helena
    Ramos Vilanova, Elayne Cristina
    da Costa, Antonio Felix
    Benko-Iseppon, Ana Maria
    Costa Ferreira-Neto, Jose Ribamar
    SOUTH AFRICAN JOURNAL OF BOTANY, 2024, 174 : 779 - 795
  • [24] Evaluation of the Use of the Tobacco PR-1a Promoter to Monitor Defense Gene Expression by the Luciferase Bioluminescence Reporter System
    Ono, Sachiko
    Kusama, Masahiro
    Ogura, Rieko
    Hiratsuka, Kazuyuki
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2011, 75 (09) : 1796 - 1800
  • [25] Defense to Sclerotium rolfsii in groundnut (Arachis hypogaea L.) is associated with vascular tissue compactness and expression of genes coding for pathogenesis-related (PR) proteins
    Bishi, Sujit Kumar
    Ranjan, Alok
    Pradhan, Bhubaneswar
    Manik, Suryakant
    Vakharia, Dinesh
    3 BIOTECH, 2025, 15 (02)
  • [26] Pathogenesis related protein-1 (PR-1) genes in tomato (Solanum lycopersicum L.): Bioinformatics analyses and expression profiles in response to drought stress
    Akbudak, M. Aydin
    Yildiz, Sukran
    Filiz, Ertugrul
    GENOMICS, 2020, 112 (06) : 4089 - 4099
  • [27] Expression profiling of pathogenesis-related Protein-1 (PR-1) genes from Solanum tuberosum reveals its critical role in phytophthora infestans infection
    Zaynab, Madiha
    Peng, Jiaofeng
    Sharif, Yasir
    Al-Yahyai, Rashid
    Jamil, Atka
    Hussain, Athar
    Khan, Khalid Ali
    Alotaibi, Saqer S.
    Li, Shuangfei
    MICROBIAL PATHOGENESIS, 2021, 161
  • [28] WEAK EXPRESSION OF THE PATHOGENESIS RELATED PR-B1 GENE AND LOCALIZATION OF RELATED PROTEIN DURING SYMBIOTIC ENDOMYCORRHIZAL INTERACTIONS IN TOBACCO ROOTS
    GIANINAZZIPEARSON, V
    TAHIRIALAOUI, A
    ANTONIW, JF
    GIANINAZZI, S
    DUMAS, E
    ENDOCYTOBIOSIS AND CELL RESEARCH, 1992, 8 (2-3): : 177 - 185
  • [29] Genome-wide analysis of pathogenesis-related protein-1 (PR-1) genes from Qingke (Hordeum vulgare L. var. nudum) reveals their roles in stress responses
    Yin, Wei
    Bai, Yuhai
    Wang, Shuai
    Xu, Kai
    Liang, Jian
    Shang, Qianhan
    Sa, Wei
    Wang, Le
    HELIYON, 2023, 9 (04)
  • [30] Pathogenesis-related protein 1 (PR-1) genes in soybean: Genome-wide identification, structural analysis and expression profiling under multiple biotic and abiotic stresses
    Almeida-Silva, Fabricio
    Venancio, Thiago M.
    GENE, 2022, 809
1234