Site-directed spin labeling of proteins for distance measurements in vitro and in cells

被引：74

作者：

Roser, P. ^{[1
,2
]}

Schmidt, M. J. ^{[1
,2
]}

Drescher, M. ^{[1
,2
]}

Summerer, D. ^{[3
]}

机构：

[1] Univ Konstanz, Dept Chem, Zukunftskolleg, Univ Str 10, D-78457 Constance, Germany

[2] Univ Konstanz, Konstanz Res Sch Chem Biol, Univ Str 10, D-78457 Constance, Germany

[3] Tech Univ Dortmund, Dept Chem & Chem Biol, Otto Hahn Str 6, D-44227 Dortmund, Germany

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2016年 / 14卷 / 24期

关键词：

ELECTRON-ELECTRON RESONANCE; ESCHERICHIA-COLI-CELLS; UNNATURAL AMINO-ACID; EPR SPECTROSCOPY; SIDE-CHAINS; PULSED EPR; RELAXATION-ENHANCEMENT; CONFORMATIONAL-CHANGES; MEMBRANE TRANSPORTER; TRITYL RADICALS;

D O I：

10.1039/c6ob00473c

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy allows studying the structure, dynamics, and interactions of proteins via distance measurements in the nanometer range. We here give an overview of available spin labels, the strategies for their introduction into proteins, and the associated potentials for protein structural studies in vitro and in the context of living cells.

引用

收藏

页码：5468 / 5476

页数：9

相关论文

共 105 条

[1] Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-L-phenylalanine [J].

Abdelkader, E. H. ;

Feintuch, A. ;

Yao, X. ;

Adams, L. A. ;

Aurelio, L. ;

Graham, B. ;

Goldfarb, D. ;

Otting, G. .

CHEMICAL COMMUNICATIONS, 2015, 51 (88) :15898-15901

[2] A New Gd3+ Spin Label for Gd3+-Gd3+ Distance Measurements in Proteins Produces Narrow Distance Distributions [J].

Abdelkader, Elwy H. ;

Lee, Michael D. ;

Feintuch, Akiva ;

Cohen, Marie Ramirez ;

Swarbrick, James D. ;

Otting, Gottfried ;

Graham, Bim ;

Goldfarb, Daniella .

JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 2015, 6 (24) :5016-5021

[3] Accessibility of nitroxide side chains: Absolute Heisenberg exchange rates from power saturation EPR [J].

Altenbach, C ;

Froncisz, W ;

Hemker, R ;

Mchaourab, H ;

Hubbell, WL .

BIOPHYSICAL JOURNAL, 2005, 89 (03) :2103-2112

[4] TRANSMEMBRANE PROTEIN-STRUCTURE - SPIN LABELING OF BACTERIORHODOPSIN MUTANTS [J].

ALTENBACH, C ;

MARTI, T ;

KHORANA, HG ;

HUBBELL, WL .

SCIENCE, 1990, 248 (4959) :1088-1092

[5] STRUCTURAL STUDIES ON TRANSMEMBRANE PROTEINS .2. SPIN LABELING OF BACTERIORHODOPSIN MUTANTS AT UNIQUE CYSTEINES [J].

ALTENBACH, C ;

FLITSCH, SL ;

KHORANA, HG ;

HUBBELL, WL .

BIOCHEMISTRY, 1989, 28 (19) :7806-7812

[6]

[Anonymous], 1976, SPIN LABELING THEORY

[7] EPR and DNP properties of certain novel single electron contrast agents intended for oximetric imaging [J].

Ardenkjær-Larsen, JH ;

Laursen, I ;

Leunbach, I ;

Ehnholm, G ;

Wistrand, LG ;

Petersson, JS ;

Golman, K .

JOURNAL OF MAGNETIC RESONANCE, 1998, 133 (01) :1-12

[8] Evaluation of spin labels for in-cell EPR by analysis of nitroxide reduction in cell extract of Xenopus laevis oocytes [J].

Azarkh, Mykhailo ;

Okle, Oliver ;

Eyring, Philipp ;

Dietrich, Daniel R. ;

Drescher, Malte .

JOURNAL OF MAGNETIC RESONANCE, 2011, 212 (02) :450-454

[9] Long-Range Distance Determination in a DNA Model System inside Xenopus laevis Oocytes by In-Cell Spin-Label EPR [J].

Azarkh, Mykhailo ;

Okle, Oliver ;

Singh, Vijay ;

Seemann, Isabelle T. ;

Hartig, Joerg S. ;

Dietrich, Daniel R. ;

Drescher, Malte .

CHEMBIOCHEM, 2011, 12 (13) :1992-1995

[10] In-Situ Spin Labeling of His-Tagged Proteins: Distance Measurements under In-Cell Conditions [J].

Baldauf, Christoph ;

Schulze, Katrin ;

Lueders, Petra ;

Bordignon, Enrica ;

Tampe, Robert .

CHEMISTRY-A EUROPEAN JOURNAL, 2013, 19 (41) :13714-13719

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