The change of gut microbiota-derived short-chain fatty acids in diabetic kidney disease

被引:24
作者
Zhong, Chenyu [1 ,2 ]
Dai, Zhiwei [1 ,2 ]
Chai, Lingxiong [1 ,2 ]
Wu, Lingping [1 ,2 ]
Li, Jianhui [2 ,3 ]
Guo, Weiying [2 ,3 ]
Zhang, Jie [2 ,3 ]
Zhang, Qun [2 ,3 ]
Xue, Congping [1 ,2 ]
Lin, Haixue [1 ,2 ]
Luo, Qun [1 ,2 ]
Cai, Kedan [1 ,2 ]
机构
[1] Univ Chinese Acad Sci, HwaMei Hosp, Dept Nephrol, Ningbo, Peoples R China
[2] Univ Chinese Acad Sci, Ningbo Inst Life & Hlth Ind, Ningbo, Peoples R China
[3] Univ Chinese Acad Sci, HwaMei Hosp, Dept Endocrinol, Ningbo, Peoples R China
关键词
acetate; butyrate; diabetes mellitus; diabetic kidney disease; gastrointestinal microbiome; propionate; short-chain fatty acids; SIZED POLYETHYLENE-GLYCOLS; SODIUM-BUTYRATE; RECEPTOR; NEPHROPATHY; DISRUPTION; DYSBIOSIS; PROTEIN;
D O I
10.1002/jcla.24062
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Background Previous studies found the dysbiosis of intestinal microbiota in diabetic kidney disease (DKD), especially the decreased SCFA-producing bacteria. We aimed to investigate the concentration of the stool and serum short-chain fatty acids (SCFAs), gut microbiota-derived metabolites, in individuals with DKD and reveal the correlations between SCFAs and renal function. Methods A total of 30 participants with DKD, 30 participants with type 2 diabetes mellitus (DM), and 30 normal controls (NC) in HwaMei Hospital were recruited from 1/1/2018 to 12/31/2019. Participants with DKD were divided into low estimated glomerular filtration rate (eGFR)(eGFR<60ml/min, n=14) and high eGFR (eGFR >= 60ml/min, n=16) subgroups. Stool and serum were measured for SCFAs with gas chromatograph-mass spectrometry. Results The DKD group showed markedly lower levels of fecal acetate, propionate, and butyrate versus NC (p<0.001, p<0.001, p=0.018, respectively) [1027.32(784.21-1357.90)]vs[2064.59(1561.82-2637.44)]mu g/g,[929.53(493.65-1344.26)]vs[1684.57(1110.54-2324.69)]mu g/g,[851.39(409.57-1611.65)] vs[1440.74(1004.15-2594.73)]mu g/g, respectively, and the lowest fecal total SCFAs concentration among the groups. DKD group also had a lower serum caproate concentration than that with diabetes (p=0.020)[0.57(0.47-0.61)]vs[0.65(0.53-0.79)]mu mol/L. In the univariate regression analysis, fecal and serum acetate correlated with eGFR (OR=1.013, p=0.072; OR=1.017, p=0.032). The correlation between serum total SCFAs and eGFR showed statistical significance (OR=1.019, p=0.024) unadjusted and a borderline significance (OR=1.024, p=0.063) when adjusted for Hb and LDL. The decrease in serum acetate and total SCFAs were found of borderline significant difference in both subgroups (p=0.055, p=0.050). Conclusion This study provides evidence that in individuals with DKD, serum and fecal SCFAs levels (fecal level in particular) were lowered, and there was a negative correlation between SCFAs and renal function.
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页数:11
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