How enzymes adapt: lessons from directed evolution

被引：285

作者：

Arnold, FH ^{[1
]}

Wintrode, PL

Miyazaki, K

Gershenson, A

机构：

[1] CALTECH, Div Chem & Chem Engn 210 41, Pasadena, CA 91125 USA

[2] Natl Inst Biosci & Human Technol, Tsukuba, Ibaraki 3058566, Japan

[3] Univ Illinois, Loomis Lab Phys, Urbana, IL 61801 USA

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 2001年 / 26卷 / 02期

关键词：

D O I：

10.1016/S0968-0004(00)01755-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Enzymes that are adapted to widely different temperature niches are being used to investigate the molecular basis of protein stability and enzyme function. However, natural evolution is complex: random noise, historical accidents and ignorance of the selection pressures at work during adaptation all cloud comparative studies. Here, we review hew adaptation in the laboratory by directed evolution can complement studies of natural enzymes in the effort to understand stability and function. Laboratory evolution experiments can attempt to mimic natural evolution and identify different adaptive mechanisms. However, laboratory evolution might make its biggest contribution in explorations of nonnatural functions, by allowing us to distinguish the properties nutured by evolution from those dictated by the laws of physical chemistry.

引用

页码：100 / 106

页数：7

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