A facile and effective synthesis of dinucleotide 5′-triphosphates

被引：14

作者：

Abramova, Tatiana V.

Vasileva, Svetlana V.

Serpokrylova, Inna Yu.

Kless, Hadar

Silnikov, Vladimir N.

机构：

[1] Inst Chem Biol & Fundamental Med, Novosibirsk 630090, Russia

[2] NuAce Technol Ltd, IL-76474 Rehovot, Israel

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2007年 / 15卷 / 20期

基金：

俄罗斯基础研究基金会;

关键词：

triphosphates; oligonucleotides; terminal phosphate activation;

D O I：

10.1016/j.bmc.2007.07.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report on the successful synthetic procedure for the conversion of 5 '-monophosphorylated 2 '-deoxydinucleotides into their 5 '-triphosphate derivatives in satisfactory to excellent yields. The activation of the terminal phosphate group was achieved under the Mukaiyama conditions in the presence of a nucleophilic catalyst. The reaction conditions (solvent, counter ions, activation time and reagent excess) were optimized for all dinucleotides. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：6549 / 6555

页数：7

共 20 条

[1] Synthesis of morpholine nucleoside triphosphates [J].