The Effect of Different Concentrations of Mesoporous Silica Nanoparticles in Antibacterial Photodynamic Therapy

Methicillin-resistant Staphylococcus aureus (MRSA) is a common bacterial species in the human body. Because of the rapid development of the resistance mechanism to various antibiotics, different treatment methods are needed. Photodynamic therapy (PDT) is a kind of treatment that can exhibit a permanent antimicrobial effect with the help of light and light sensitive agents. As a result of light application, reactive oxygen species are formed and used to destroy bacteria cells by giving permanent damage. In this study, PDT applications were performed on MRSA by using mesoporous silica nanoparticles. The effect of positively charged mesoporous silica nanoparticles on bacterial strains was assessed simultaneously with PDT applications. According to the results, it has been seen that the use of nanoparticles reduces the survival rates in PDT applications and help to reduce the PDT parameters to minimum levels. In addition, increasing nanoparticle concentration in the range of 25-200 mg/ml did not change the survival rates after PDT applications Thus, the antibacterial efficacy of PDT was further enhanced by these nanoparticles and showed promise for in vivo and clinical applications.