Genetic analyses of Trypanosoma cruzi isolates from naturally infected triatomines and humans in northeastern Brazil

被引：36

作者：

Camara, A. C. J. ^{[2
,4
]}

Varela-Freire, A. A. ^{[4
]}

Valadares, H. M. S. ^{[3
]}

Macedo, A. M. ^{[3
]}

D'Avila, D. A. ^{[2
]}

Machado, C. R. ^{[3
]}

Lages-Silva, E. ^{[5
]}

Chiari, E. ^{[2
]}

Galvao, L. M. C. ^{[1
,2
]}

机构：

[1] Univ Fed Rio Grande do Norte, Ctr Ciencias Saude, Fac Farm, Programas Posgrad Ciencias Saude & Ciencias Farma, BR-59012570 Natal, RN, Brazil

[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Parasitol, Belo Horizonte, MG, Brazil

[3] Dept Bioquim & Imunol, BR-31270901 Belo Horizonte, MG, Brazil

[4] Univ Fed Rio Grande do Norte, Ctr Biociencias, Dept Microbiol & Parasitol, BR-59078900 Natal, RN, Brazil

[5] Univ Fed Triangulo Mineiro, Dept Ciencias Biol, BR-38025180 Uberaba, MG, Brazil

来源：

ACTA TROPICA | 2010年 / 115卷 / 03期

关键词：

Trypanosoma cruzi; Chagas disease infection; Genomic markers; Triatoma brasiliensis; Panstrongylus lutzi; T. cruzi III; CHAGAS-DISEASE CONTROL; RIBOSOMAL-RNA; MOLECULAR EPIDEMIOLOGY; POPULATION-STRUCTURE; POLYMORPHIC MICROSATELLITES; PHYLOGENETIC LINEAGES; MINI-EXON; STRAINS; DNA; FUTURE;

D O I：

10.1016/j.actatropica.2010.03.003

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Trypanosoma cruzi genetic diversity was investigated in 25 isolates (vectors and humans) from the semi-arid zone of the State of Rio Grande do Norte, Brazil. Molecular markers (3' region of the 24S alpha rRNA; mitochondrial cytochrome oxidase subunit 2 (COII) gene; spliced leader intergenic region (SL-IR) gene; allelic size microsatellite polymorphism) identified 56% TcIII (100% Panstrongylus lutzi; 50% Triatoma brasiliensis); 40% TcII (91.7% humans; 50% T. brasiliensis) and 4% TcI (human). Microsatellite analysis revealed monoclonal and heterozygous patterns on one or more microsatellite loci in 64% of T. cruzi isolates (92.3% triatomines; 33.3% humans) and 36% putative polyclonal populations (66.7% humans; 7.7% triatomines) by loci SCLE10, SCLE11, TcTAT20, TcAAAT6, all belonging to TcII. Identical T. cruzi polyclonal profiles (88.9%) were detected, mostly from humans. The adaptative natural plasticity of TcII and TcIII and their potential for maintaining human infection in T. brasiliensis were confirmed. Intraspecific and phylogenetic T. cruzi diversity in the sylvatic and domestic transmission cycles in this specific region will provide exclusive control strategies. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：205 / 211

页数：7

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