Nanoparticle-Functionalized Porous Polymer Monolith Detection Elements for Surface-Enhanced Raman Scattering

被引：98

作者：

Liu, Jikun ^{[2
]}

White, Ian ^{[1
]}

DeVoe, Don L. ^{[1
,2
]}

机构：

[1] Univ Maryland, Fischell Dept Bioengn, College Pk, MD 20742 USA

[2] Univ Maryland, Dept Mech Engn, College Pk, MD 20742 USA

来源：

ANALYTICAL CHEMISTRY | 2011年 / 83卷 / 06期

基金：

美国国家卫生研究院;

关键词：

SINGLE-MOLECULE; GOLD NANOPARTICLES; SILVER; SPECTROSCOPY; SERS; BRADYKININ; PROTEINS; CHANNELS; CHIP; TOOL;

D O I：

10.1021/ac102932d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The wuse of porous polymer monoliths functionalized with silver nanoparticles is introduced in this work for high-sensitivity surface-enhanced Raman scattering (SERS) detection. Preparation of the SERS detection elements is a simple process comprising the synthesis of a discrete polymer monolith section within a silica capillary, followed by physically trapping silver nanoparticle aggregates within the monolith matrix. A SERS detection limit of 220 fmol for Rhodamine 6G is demonstrated, with excellent signal stability over a 24 h period. The capability of the SERS-active monolith for label-free detection of biomolecules was demonstrated by measurements of bradykinin and cyctochrome c. The SERS-active monoliths can be readily integrated into miniaturized micrototal-analysis systems for online and label-free detection for a variety of biosensing,, bioanalytical, and biomedical applications.

引用

页码：2119 / 2124

页数：6

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