Nanoparticle-Functionalized Porous Polymer Monolith Detection Elements for Surface-Enhanced Raman Scattering

被引:98
作者
Liu, Jikun [2 ]
White, Ian [1 ]
DeVoe, Don L. [1 ,2 ]
机构
[1] Univ Maryland, Fischell Dept Bioengn, College Pk, MD 20742 USA
[2] Univ Maryland, Dept Mech Engn, College Pk, MD 20742 USA
基金
美国国家卫生研究院;
关键词
SINGLE-MOLECULE; GOLD NANOPARTICLES; SILVER; SPECTROSCOPY; SERS; BRADYKININ; PROTEINS; CHANNELS; CHIP; TOOL;
D O I
10.1021/ac102932d
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The wuse of porous polymer monoliths functionalized with silver nanoparticles is introduced in this work for high-sensitivity surface-enhanced Raman scattering (SERS) detection. Preparation of the SERS detection elements is a simple process comprising the synthesis of a discrete polymer monolith section within a silica capillary, followed by physically trapping silver nanoparticle aggregates within the monolith matrix. A SERS detection limit of 220 fmol for Rhodamine 6G is demonstrated, with excellent signal stability over a 24 h period. The capability of the SERS-active monolith for label-free detection of biomolecules was demonstrated by measurements of bradykinin and cyctochrome c. The SERS-active monoliths can be readily integrated into miniaturized micrototal-analysis systems for online and label-free detection for a variety of biosensing,, bioanalytical, and biomedical applications.
引用
收藏
页码:2119 / 2124
页数:6
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