Limitations in MTBE biodegradation

被引：28

作者：

Fayolle, F ^{[1
]}

François, A ^{[1
]}

Garnier, L ^{[1
]}

Godefroy, D ^{[1
]}

Mathis, H ^{[1
]}

Piveteau, F ^{[1
]}

Monot, F ^{[1
]}

机构：

[1] IFP Energies Nouvelles, Dept Biotechnol & Chim Biomasse, F-92852 Rueil Malmaison, France

来源：

OIL & GAS SCIENCE AND TECHNOLOGY-REVUE D IFP ENERGIES NOUVELLES | 2003年 / 58卷 / 04期

关键词：

D O I：

10.2516/ogst:2003033

中图分类号：

TE [石油、天然气工业]; TK [能源与动力工程];

学科分类号：

0807 ; 0820 ;

摘要：

The methyl tert-butyl ether (MTBE) metabolic pathway was partially elucidated in Mycobacterium austroafricanum IFP 2012 by identifying the degradation intermediates. Several enzymatic activities were specifically induced during growth on MTBE. Among those required for the first steps of MTBE degradation to tent-butyl alcohol (TBA), the same monooxygenase was responsible for the oxidation of both MTBE and TBA, with a low affinity for TBA (Km = 1.1 mM). An esterase was involved in the hydrolysis of tert-butyl formate (TBF). The slowness of the degradation of MTBE by M. austroafricanum IFP 2012 was the result of complex interactions, especially the negative effect of W formed during MTBE oxidation on the MTBE/TBA moonoxygenase and the absence of TBA oxidation in the presence of residual MTBE. Moreover, concerning the downstream steps of MTBE metabolism, Cc(++) ions were required for the degradation of 2-hydroxyisobutyric acid (HIBA) formed by oxidation of TBA as shown by the low growth yield on HIBA in the absence of cobalt.

引用

页码：497 / 504

页数：8

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