Xenogeneic chimera-Generated by blastocyst complementation-As a potential unlimited source of recipient-tailored organs

被引：14

作者：

Oldani, Graziano ^{[1
,2
]}

Peloso, Andrea ^{[1
,3
]}

Lacotte, Stephanie ^{[1
]}

Meier, Raphael ^{[1
,2
]}

Toso, Christian ^{[1
,2
]}

机构：

[1] Univ Geneva, Div Abdominal Surg, Dept Surg, Fac Med, Geneva, Switzerland

[2] Geneva Univ Hosp, Hepatopancreatobiliary Ctr, Geneva, Switzerland

[3] Univ Pavia, Div Gen Surg, IRCCS Policlin San Matteo Fdn, Pavia, Italy

来源：

XENOTRANSPLANTATION | 2017年 / 24卷 / 04期

关键词：

blastocyst; chimera; complementation; organ; xenotransplantation; PLURIPOTENT STEM-CELLS; LIVER-TRANSPLANTATION; ETHICAL-ISSUES; KNOCKOUT MICE; IN-VIVO; XENOTRANSPLANTATION; MOUSE; PANCREAS; KIDNEY; RAT;

D O I：

10.1111/xen.12327

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Blastocyst complementation refers to the injection of cells into a blastocyst. The technology allows for the creation of chimeric animals, which have the potential to be used as an unlimited source of organ donors. Pluripotent stem cells could be generated from a patient in need of a transplantation and injected into a large animal blastocyst (potentially of a pig), leading to the creation of organ(s) allowing immunosuppression-free transplantation. Various chimera combinations have already been generated, but one of the most recent steps leads to the creation of human-pig chimeras, which could be studied at an embryo stage. Although still far from clinical reality, the potential application is almost unlimited. The present review illustrates the historical steps of intra- and interspecific blastocyst complementation in rodents and large animals, specifically looking at its potential for generation of organ grafts. We also speculate on how it could change transplant indications, on its economic impact, and on the linked ethical concerns.

引用

页数：6

共 49 条

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