Characterization of Fusarium spp. isolates recovered from bananas (Musa spp.) affected by Fusarium wilt in Puerto Rico

被引:15
作者
Garcia, Raymond O. [1 ]
Rivera-Vargas, Lydia I. [1 ]
Ploetz, Randy [2 ]
Correll, James C. [3 ]
Irish, Brian M. [4 ,5 ]
机构
[1] Univ Puerto Rico, Dept Agroenvironm Sci, Mayaguez, PR USA
[2] Univ Florida, Ctr Trop Res & Educ, Homestead, FL 33031 USA
[3] Univ Arkansas, Dept Plant Pathol, Fayetteville, AR 72701 USA
[4] USDA ARS, Trop Agr Res Stn, Mayaguez, PR 00682 USA
[5] USDA ARS Prosser, Plant Germplasm Intro Testing & Res Unit, Pullman, WA USA
关键词
Fusarium; Musa; Banana; Vegetative compatibility group; F-SP CUBENSE; TROPICAL RACE 4; VEGETATIVE COMPATIBILITY; OXYSPORUM; DIAGNOSIS; PATHOGEN;
D O I
10.1007/s10658-018-1503-y
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense (FOC), is one of the most destructive diseases of bananas (Musa spp.) worldwide. Although the first report of this disease in Puerto Rico was in 1919, little has been published since that time. We report the first systematic description of FOC on the island. A total of 28 single-spore isolates of Fusarium spp. were recovered from symptomatic bananas from several municipalities. Species identity of the isolates was initially based on morphology and later confirmed with partial sequences of the translation elongation factor 1 alpha (TEF-1 alpha) gene. Isolates were examined for vegetative compatibility, race identity using PCR primers reported for different races, and virulence on tissue-culture-derived plantlets of differential cultivars for races 1, 2 and 4. Most isolates of F. oxysporum belonged to VCG 0124 of FOC, and amplified with primers designed for race 1. Several isolates amplified with primers developed for race 4, but no isolate amplified with primers specific for tropical race 4 (TR4). Most isolates produced symptoms on race 1 or race 2 susceptible cultivars. Although some isolates caused minor symptoms on Gran Nain', disease severities on this race 4 differential were considerably lower than those in susceptible race 1 or race 2 interactions. Based on these results, race 1 and race 2, but not TR4, are present in Puerto Rico. We discuss the disease and FOC's population structure in Puerto Rico, as well as the reliability of different PCR primers that have been developed to identify races of this pathogen.
引用
收藏
页码:599 / 611
页数:13
相关论文
共 39 条
[1]   Development of a hydrolysis probe-based real-time assay for the detection of tropical strains of Fusarium oxysporum f. sp cubense race 4 [J].
Aguayo, Jaime ;
Mostert, Diane ;
Fourrier-Jeandel, Celine ;
Cerf-Wendling, Isabelle ;
Hostachy, Bruno ;
Viljoen, Altus ;
Ioos, Renaud .
PLOS ONE, 2017, 12 (02)
[2]  
[Anonymous], 1966, BANANAS
[3]  
[Anonymous], TRAINERS MANUAL TRAI
[4]  
[Anonymous], 2015, ASSESSMENT END END S
[5]  
Armitage A.D., 2017, BIORXIV, DOI [10.1101/182238, DOI 10.1101/182238]
[6]  
Bancroft J., 1876, Votes and Proceedings, P1011
[7]  
Brandes EW, 1919, PHYTOPATHOLOGY, V9, P339
[8]   NITRATE NON-UTILIZING MUTANTS OF FUSARIUM-OXYSPORUM AND THEIR USE IN VEGETATIVE COMPATIBILITY TESTS [J].
CORRELL, JC ;
KLITTICH, CJR ;
LESLIE, JF .
PHYTOPATHOLOGY, 1987, 77 (12) :1640-1646
[9]  
Cortes M, 2006, J AGR U PUERTO RICO, V90, P3
[10]   Investigation of the diversity of effector genes in the banana pathogen, Fusarium oxysporum f. sp cubense, reveals evidence of horizontal gene transfer [J].
Czislowski, Elizabeth ;
Fraser-Smith, Sam ;
Zander, Manuel ;
O'Neill, Wayne T. ;
Meldrum, Rachel A. ;
Tran-Nguyen, Lucy T. T. ;
Batley, Jacqueline ;
Aitken, Elizabeth A. B. .
MOLECULAR PLANT PATHOLOGY, 2018, 19 (05) :1155-1171