Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants

被引：20

作者：

Bai, Jie-Ying

Zeng, Lin

Hu, Yuan-Lei ^{[1
]}

Li, Yan-Fang

Lin, Zhong-Ping

Shang, Shi-Chen

Shi, Yan-Sheng

机构：

[1] Peking Univ, Natl Lab Protein Engn & Plant Genet Engn, Beijing 100871, Peoples R China

[2] Acad Mil Med Sci, Lab Anim Ctr, Beijing 100071, Peoples R China

[3] Jilin Univ, Coll Plant Sci, Changchun 130062, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 12期

关键词：

biological activity; human epidermal growth factor; matrix attachment regions; optimized codons; tobacco;

D O I：

10.1007/s10529-007-9438-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To improve the accumulation of recombinant human epidermal growth factor (hEGF) in transgenic tobacco, a highly effective vector was constructed and transformed via Agrobacterium tumefaciens. The hEGF content in transgenic tobacco was up to 0.3% of the total soluble protein. Using the Vero E6 cell expansion assay and the MTT method for cell proliferation, hEGF produced by transgenic tobacco significantly stimulated Vero E6 cell expansion and proliferation, the same as commercial hEGF products.

引用

页码：2007 / 2012

页数：6

共 18 条

[1] Compartment-specific accumulation of recombinant immunoglobulins in plant cells: an essential tool for antibody production and immunomodulation of physiological functions and pathogen activity [J].