Universal indirect enzyme-linked immunosorbent assay for monitoring of human and animal brucellosis in Kazakhstan

被引：7

作者：

Grushina, T.

Atshabar, B.

Syzdykov, M.

Daulbaeva, S.

Tserelson, L.

Kuznetsov, A.

Baramova, Sh.

Seidakhmetova, R.

Sultanov, A.

Ospanov, Y.

Mikhalev, A.

Amireev, S.

Ospanov, K.

Kazakov, S.

Mizanbayeva, S.

Myrzabekov, A.

Rementsova, M.

Berezovskiy, D.

Akasheva, R.

Khasenov, M.

Nussipova, Zh.

Yu, W.

Nielsen, K.

机构：

[1] Animal Diseases Research Institute, Canadian Food Inspection Agency

来源：

VACCINE | 2010年 / 28卷

关键词：

Human and animal brucellosis; SAT; RBT; CFT; iELISA; Brucella melitensis; DIAGNOSIS; IMMUNOASSAY;

D O I：

10.1016/j.vaccine.2010.03.052

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Combinations of conventional serological methods and new ELISA procedures were evaluated to develop the most efficient and effective diagnostics for the detection of brucellosis in humans and animals. Sera from humans (n = 249) and animals (n = 99) were collected from brucellosis endemic areas (Zhambyl district and Enbekshi-Kazakh district of Almaty region in Kazakhstan) for serologic analysis. Sera from the humans reacted positively in the RBT (38.5%), SAT (43.3%), iELISA (42.5%) while sera from the animals reacted positively in RBT (79.8%), SAT (89.9%), CF (87.8%), iELISA (100%). Greater seropositivity was detected in animals as compared to human samples. All positive sera were also evaluated on an indirect ELISA (iELISA). Bacterial isolation was attempted on seropositive human sera. Our data indicate that the combination of conventional serological tests (SAT and CF), combined with the iELISA is optimal for the processing of large numbers of samples and the most efficient detection of human and animal brucellosis. (C) 2010 Published by Elsevier Ltd.

引用

页码：F46 / F48

页数：3

共 9 条

[1]

Corbel M.J., 2006, BRUCELLOSIS HUMANS A, P31

[2]

*FAO WHO EXP COMM, 1986, TECHN REP SER FAO WH, V740, P133

[3]

GRUSHINA T, 2003, BRUC 2003 INT RES C, P108

[4]

GRUSHINA TA, 2006, ALMATY B KAZAKH NATL, V4, P111

[5] Competitive enzyme immunoassay for diagnosis of human brucellosis [J].

Lucero, NE ;

Foglia, L ;

Ayala, SM ;

Gall, D ;

Nielsen, K .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (10) :3245-3248

[6] Fluorescence polarization assay for diagnosis of human brucellosis [J].

Lucero, NE ;

Escobar, GI ;

Ayala, SM ;

Paulo, PS ;

Nielsen, K .

JOURNAL OF MEDICAL MICROBIOLOGY, 2003, 52 (10) :883-887

[7] Fluorescence polarization immunoassay:: Detection of antibody to Brucella abortus [J].

Nielsen, K ;

Lin, M ;

Gall, D ;

Jolley, M .

METHODS-A COMPANION TO METHODS IN ENZYMOLOGY, 2000, 22 (01) :71-76

[8] Enzyme immunoassay for the diagnosis of brucellosis: chimeric Protein A-Protein G as a common enzyme labeled detection reagent for sera for different animal species [J].

Nielsen, K ;

Smith, P ;

Yu, W ;

Nicoletti, P ;

Elzer, P ;

Vigliocco, A ;

Silva, P ;

Bermudez, R ;

Renteria, T ;

Moreno, F ;

Ruiz, A ;

Massengill, C ;

Muenks, Q ;

Kenny, K ;

Tollersrud, T ;

Samartino, L ;

Conde, S ;

de Benitez, GD ;

Gall, D ;

Perez, B ;

Rojas, X .

VETERINARY MICROBIOLOGY, 2004, 101 (02) :123-129

[9] The use of homologous antigen in the serological diagnosis of brucellosis caused by Brucella melitensis [J].

Tittarelli, M ;

Giovannini, A ;

Conte, A ;

Di Ventura, M ;

Nannini, D ;

Caporale, V .

JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 2005, 52 (02) :75-81

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