Overexpression of C/EBP Affects The Cell Cycle Regulators and Spermatogenesis Related Genes Expression And Function of Bovine Sertoli Cells

被引：4
作者：
Tang, K. [1 ]
Jin, Y. [1 ]
Chen, F. [1 ]
Wang, L. [1 ]
机构：
[1] Northwest A&F Univ, Coll Vet Med, Xianyang 712100, Shaanxi, Peoples R China
来源：
REPRODUCTION IN DOMESTIC ANIMALS | 2016年 / 51卷 / 04期
关键词：
GRANULOSA-CELLS; BETA; APOPTOSIS; PROGESTERONE; TESTIS; DIFFERENTIATION; PROLIFERATION; EXPANSION; PROTEINS; BARRIER;
D O I：
10.1111/rda.12724
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Contents CCAAT/enhancer-binding protein beta (C/EBP), an important transcriptional factor, plays a pivotal role in the regulation of female germ cell development. However, the role of C/EBP on the development of male germ cells has not been reported. In this study, we constructed the recombinant adenovirus plasmids of bovine C/EBP gene and harvested the subsequent adenoviruses, and then assessed the mRNA levels of spermatogenesis-related genes (real-time PCR) and secretion of inhibin B after 48h of Ad-C/EBP recombinant adenovirus infection in bovine sertoli cells (SCs). We found that overexpression of exogenous C/EBP gene upregulated the mRNA expressions of spermatogenesis-related genes, including Pdgfa, Claudin, Caspase-3, Occludin, kit1 and Cyclin E, and decreased the mRNA levels of Cyclin D1. Meanwhile, overexpression of exogenous C/EBP gene significantly increases the amounts of secreted inhibin B. In conclusion, the results indicate that the C/EBP gene plays an important regulatory role in regulation of the cell cycle regulators and spermatogenesis-related genes expression and function of bovine SCs.
引用
收藏
页码：591 / 596
页数：6
相关论文
共 33 条
  • [1] The C/EBPbeta isoform, liver-inhibitory protein (LIP), induces autophagy in breast cancer cell lines
    Abreu, Maria M.
    Sealy, Linda
    [J]. EXPERIMENTAL CELL RESEARCH, 2010, 316 (19) : 3227 - 3238
  • [2] Causes and consequences of apoptosis in spermatozoa; contributions to infertility and impacts on development
    Aitken, R. John
    Baker, Mark A.
    [J]. INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, 2013, 57 (2-4) : 265 - 272
  • [3] Alao JP, 2006, MOL CANCER, V5, P11
  • [4] One tissue, two fates: Molecular genetic events that underlie testis versus ovary development
    Brennan, J
    Capel, B
    [J]. NATURE REVIEWS GENETICS, 2004, 5 (07) : 509 - 521
  • [5] Action Mechanism of Inhibin α-Subunit on the Development of Sertoli Cells and First Wave of Spermatogenesis in Mice
    Cai, Kailai
    Hua, Guohua
    Ahmad, Sibtain
    Liang, Aaixin
    Han, Li
    Wu, Canjie
    Yang, Feifei
    Yang, Liguo
    [J]. PLOS ONE, 2011, 6 (10):
  • [6] Fanning AS, 1999, J AM SOC NEPHROL, V10, P1337
  • [7] Critical role for transcription factor C/E.BP-β in regulating the expression of death-associated protein kinase 1
    Gade, Padmaja
    Roy, Sanjit K.
    Li, Hui
    Nallar, Shreeram C.
    Kalvakolanu, Dhananjaya V.
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 2008, 28 (08) : 2528 - 2548
  • [8] The role of C/EBPβ in mammary gland development and breast cancer
    Grimm, SL
    Rosen, JM
    [J]. JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA, 2003, 8 (02) : 191 - 204
  • [9] Characterization of the Mechanism of Inhibin α-Subunit Gene in Mouse Anterior Pituitary Cells by RNA Interference
    Han, Li
    Wu, Canjie
    Riaz, Hasan
    Bai, Liya
    Chen, Jianguo
    Zhen, Yanhong
    Guo, Aizhen
    Yang, Liguo
    [J]. PLOS ONE, 2013, 8 (10):
  • [10] Testicular germ cell apoptosis and formation of the sertoli cell barrier during the initiation of spermatogenesis in pubertal stallions
    Heninger, N. L.
    Staub, C.
    Johnson, L.
    Blanchard, T. L.
    Varner, D.
    Ing, N. H.
    Forrest, D. W.
    [J]. ANIMAL REPRODUCTION SCIENCE, 2006, 94 (1-4) : 127 - 131
1234