miR-200c affects the mRNA expression of E-cadherin by regulating the mRNA level of TCF8 during post-natal epididymal development in juvenile rats

被引：14

作者：

Wang, Junfeng ^{[1
]}

Ruan, Kangcheng ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2010年 / 42卷 / 09期

关键词：

epididymis; miR-200c; TCF8; E-cadherin; interleukin-2; C-ELEGANS; CELLS; MICRORNAS; DICER; GENE; DIFFERENTIATION; MORPHOGENESIS; HSA-MIR-200C; MICROARRAY; PCR;

D O I：

10.1093/abbs/gmq073

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The unique temporal expression pattern of miR-200c in epididymis during post-natal development in juvenile rats was revealed by our home-made miRNA microarray in this paper. It was found that miR-200c expressed in the lowest level at Day 7 and then increased to the highest at Day 36 followed by a dramatic decrease. The pattern was exactly inverse to that of mRNA expression of transcription factor 8 (TCF8) revealed by quantitative real-time polymerase chain reaction (qRT-PCR), providing an extra evidence that TCF8 is one degradation target of miR-200c even in epididymis. Moreover, the qRT-PCR study on expression of E-cadherin and interleukin-2 indicated that miR-200c does exert an obvious effect on the mRNA expression of E-cadherin by directly regulating the mRNA level of TCF8, although the effect on interleukin-2 is not obvious as on E-cadherin, which implicates that interleukin-2 may be also regulated by other factors besides TCF8 in rat epididymis.

引用

页码：628 / 634

页数：7

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