KIOM-79 prevents methyglyoxal-induced retinal pericyte apoptosis in vitro and in vivo

被引:24
作者
Kim, Ohn Soon [1 ]
Kim, Junghyun [1 ]
Kim, Chan-Sik [1 ]
Kim, Nan Hee [1 ]
Kim, Jin Sook [1 ]
机构
[1] Korea Inst Oriental Med, Diabet Complicat Res Ctr, Div Tradit Korean Med Integrated Res, Taejon 305811, South Korea
关键词
KIOM-79; Diabetic retinopathy; Retinal pericyte; Apoptosis; Oxidative stress; METHYLGLYOXAL-INDUCED APOPTOSIS; DIABETIC-RETINOPATHY; HIGH GLUCOSE; AGES ACCUMULATION; ENDOTHELIAL-CELLS; OXIDATIVE STRESS; AMINO-ACIDS; RAT RETINA; ACTIVATION; EXPRESSION;
D O I
10.1016/j.jep.2010.03.027
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Aims of study: The purpose of this study was to investigate the protective effects of KIOM-79, a combination of four plant extracts, on retinal pericytes loss, which is one of the histopathological hallmarks of early diabetic retinopathy. Materials and methods: To investigate the protective effect of KIOM-79 on pericyte apoptosis, we have used methylglyoxal (MGO)-treated primary rat retinal pericytes and retinal vessels from intravitreally MGO-injected eyes. Primary rat retinal pericytes were exposed to 400 mu M MGO for 6 h with or without KIOM-79. 400 mu M final intravitreal concentration of MGO with or without KIOM-79 (10 mu g/ml final intravitreal concentration) were intravitreally injected into the right eyes of rats for 2 days. The left eyes were received 3 mu l of saline only. Retinal vessels were then isolated. We examined apoptosis, ROS productions, 8-OHdG in cultured rat retinal pericytes and retinal vessels. Results: In CCK-8 assay and TUNEL staining, MGO-induced apoptosis of rat retinal pericytes was markedly inhibited by KIOM-79. KIOM-79 significantly reduced intracellular ROS productions and oxidative damage induced by MGO. In addition, the intravitreal injection of KIOM-79 prevented apoptosis of retinal microvascular cells, MGO accumulation and oxidative DNA damage in intravitreally MGO-injected eye. Conclusion: These observations suggest that KIOM-79 acts through an antioxidant mechanism to protect against oxidative stress-induced apoptosis in retinal pericytes. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:285 / 292
页数:8
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