Effect of Silane-Coupling Agent Concentration on Morphology and In Vitro Bioactivity of Gelatin-Based Nanofibrous Scaffolds Fabricated by Electrospinning Method

In the present study, gelatin-based hybrid fibrous scaffolds with and without silane agent (Epoxypropoxy-propyl-trimethoxysilane) were synthesized by electrospinning method. The effect of different concentrations of silane agent on morphology of the scaffolds was investigated. Scanning electron microscope observations showed that using the agent into the gelatin solution induced formation of fibers with appropriate uniformity with respect to the other samples. Nitrogen adsorption test revealed that there was no pore inside the fibers and surface area data were related to spaces between fibers. Physical analyses depicted that these kinds of fibers were linked to the silane agent by the covalent bond. Different morphologies of apatite were occurred on surface of the fibers after various days of soaking in simulated body fluid. Fourier transmission infrared spectroscopy and X-ray confirmed apatite formation on the fiber surface as well. Pore size distribution and specific surface area of these fibers after soaking nearly changed compared to the samples before soaking. Evaluation of cell viability and cell growth demonstrated that human osteoblast-like cells were attached, spread and grown well on the surface. This event could be a good sign of biocompatibility of the fibers.