Lipid diffusion in the thylakoid membranes of the cyanobacterium Synechococcus sp.:: effect of fatty acid desaturation

被引：34
作者：
Sarcina, M
Murata, N
Tobin, MJ
Mullineaux, CW
机构：
[1] UCL, Dept Biol, London WC1E 6BT, England
[2] Natl Inst Basic Biol, Okazaki, Aichi 444, Japan
[3] SERC, Daresbury Lab, CLRC, Warrington WA4 4AD, Cheshire, England
来源：
FEBS LETTERS | 2003年 / 553卷 / 03期
基金：
英国生物技术与生命科学研究理事会;
关键词：
cyanobacterium; thylakoid membrane; photosynthesis; lipid; diffusion; membrane fluidity;
D O I：
10.1016/S0014-5793(03)01031-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Thylakoid membranes are crucial to photosynthesis in cyanobacteria and plants. In cyanobacteria, genetic modification of membrane lipid composition strongly influences cold tolerance and susceptibility to photoinhibition. We have used fluorescence recovery after photobleaching to measure the diffusion of a lipid-soluble fluorescent marker in cells of the cyanobacterium Synechococcus sp. PCC 7942. We have compared the wild-type strain with a transformant with an increased level of fatty acid unsaturation. The transformant showed a six-fold increase in the diffusion coefficient for the fluorescent marker at growth temperature. This is the first direct measurement of lipid diffusion in a photosynthetic membrane. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：295 / 298
页数：4
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