Immobilization of Candida rugosa lipase on chitosan with activation of the hydroxyl groups

被引：361

作者：

Chiou, SH ^{[1
]}

Wu, WT ^{[1
]}

机构：

[1] Natl Tsing Hua Univ, Dept Chem Engn, Hsinchu 30043, Taiwan

来源：

BIOMATERIALS | 2004年 / 25卷 / 02期

关键词：

Candida rugosa lipase; chitosan; immobilization; activation; swelling;

D O I：

10.1016/S0142-9612(03)00482-4

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A method for immobilization of Candida rugosa lipase to two types of chitosan beads by activating the hydroxyl groups of chitosan using carbodiimide coupling agent has been successfully developed. The ability of carbodiimide to activate the hydroxyl groups of chitosan was confirmed using the electron spectroscopy for chemical analysis (ESCA) technique. The properties of lipase immobilized using dry and wet chitosan beads were also investigated and compared. Immobilization enhanced the enzyme stability against changes of pH and temperature. High storage stability of 30 days and an increased enzyme activity of 2,110% were observed in wet immobilized lipase. Immobilized lipase using dry and wet chitosan beads retained 78% and 85% of its initial activity after 10 batch hydrolytic cycles. The kinetic parameters K-m and V-max were determined for the free and immobilized lipase. The activation energy (E-a) was found to decrease for immobilization of lipase on chitosan beads. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：197 / 204

页数：8

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