Characterization of two related exoantigens from the biodeteriogenic fungus Aspergillus versicolor

被引:17
作者
Liang, Yinan [1 ]
Zhao, Wendy [1 ]
Xu, Jianping [1 ]
Miller, J. David [1 ]
机构
[1] Carleton Univ, Dept Chem, Ottawa, ON K1S 5B6, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
43 kDa exoantigen; Aspergillus versicolor; Biodeterioraton; Monoclonal antibodies; ALKALINE SERINE PROTEINASE; HYPERSALINE DEAD-SEA; STACHYBOTRYS-CHARTARUM; BUILDING-MATERIALS; MONOCLONAL-ANTIBODIES; FILAMENTOUS FUNGI; SEQUENCE ANALYSIS; QUANTITATIVE PCR; MAJOR ALLERGEN; MYCOTOXINS;
D O I
10.1016/j.ibiod.2010.11.005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aspergillus versicolor is one of the most common fungi in damp buildings in U. K., various European and Scandinavian countries as well as the United States and Canada. It is a proxy for species that occur at similar material water activities. Based on studies from Finland. Norway and Germany, it is among the common species resulting in an IgE reaction. Using pre-screened human sera with antibodies to various fungi, two related proteins were discovered with molecular weights 43 and 41 kDa based on SDS electrophoresis. Both proteins were excreted on the surfaces of spores and into culture media. The 41 kDa protein has a pI of 4.5. Based on a partial sequence, it is a serine protease. There are a number of Aspergillus proteases with overlapping sequences but these have different molecular weights and pI values. Polyclonal and monoclonal antibodies were developed that were specific compared to a diverse taxonomic array of related and unrelated fungi that commonly occur in the built environment. Initially this was done to ensure the specificity of the target protein. The measurement of other allergens and antigens associated with the built environment has a number of uses. Most importantly, these can be used to assess reliably biodeterioration and contribute to improved exposure assessments for population health studies. (C) 2010 Published by Elsevier Ltd.
引用
收藏
页码:217 / 226
页数:10
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