Genetic Divergence between Auxis thazard and A. rochei based on PCR-RFLP Analysis of mtDNA D-loop Region

被引：5

作者：

Kumar, Girish ^{[1
,2
]}

Kunal, Swaraj Priyaranjan ^{[1
]}

Menezes, Maria Rosalia ^{[1
]}

Kocour, Martin ^{[2
]}

机构：

[1] Natl Inst Oceanog, Div Biol Oceanog, Panaji 403004, Goa, India

[2] Univ South Bohemia Ceske Budejovice, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy, Vodnany 38925, Czech Republic

来源：

TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES | 2014年 / 14卷 / 02期

关键词：

Tuna; Genetic variation; Conservation; Restriction sites; Haplotype; POPULATION-GENETICS; DNA; PHYLOGEOGRAPHY; STOCKS;

D O I：

10.4194/1303-2712-v14_2_25

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Tunas of genus Auxis are commercially and ecologically important fish of family Scombridae. There is currently no information concerning the genetic divergence between A. thazard and A. rochei. Therefore, a study was conducted to estimate the degree of genetic divergence between two species based on PCR-RFLP analysis of D-loop region (500 bp) of mitochondrial DNA in 100 individuals collected from Tuticorin (India). High level of genetic divergence (9.26%) was observed between A. thazard and A. rochei. Estimates of molecular diversity indices were higher in A. rochei (pi = 3.8808; hd = 0.9641) compared to A. thazard (pi = 2.2514; hd = 0.8441). Based on demographic parameters, population expansion of A. thazard estimated to begin about 67,167 years before present while of founding of A. rochei took place about 114,747 years before present in Indian Ocean. Thus, we conclude that A. rochei represents the ancestral species of genus Auxis.

引用

页码：539 / 546

页数：8

共 27 条

[1]

[Anonymous], 1987, Science, Philosophy, and Human Behavior in the Soviet Union

[2] Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea [J].