Anti-Inflammatory Effect of Dehydroxymethylepoxyquinomicin, a Nuclear factor-κB Inhibitor, on Endotoxin-Induced Uveitis in Rats In vivo and In vitro

被引：14
作者：
Ando, Yoshimasa [1 ]
Keino, Hiroshi [1 ]
Kudo, Akihiko [2 ]
Hirakata, Akito [1 ]
Okada, Annabelle A. [1 ]
Umezawa, Kazuo [3 ]
机构：
[1] Kyorin Univ, Sch Med, Dept Ophthalmol, 6-20-2 Shinkawa, Mitaka, Tokyo 1818611, Japan
[2] Kyorin Univ, Sch Med, Dept Anat, Mitaka, Tokyo, Japan
[3] Aichi Med Univ, Dept Mol Target Med, Nagakute, Aichi, Japan
来源：
OCULAR IMMUNOLOGY AND INFLAMMATION | 2020年 / 28卷 / 02期
基金：
日本学术振兴会;
关键词：
Dexamethasone; DHMEQ; endotoxin-induced uveitis (EIU); NF-kappa B inhibitor; peritoneal exudate cells (PEC); TUMOR-NECROSIS-FACTOR; PIGMENT EPITHELIAL-CELLS; SMALL-MOLECULE INHIBITOR; BACTERIAL-ENDOTOXIN; GENE-EXPRESSION; DHMEQ; EPOXYQUINOMICIN; INFLAMMATION; ACTIVATION; APOPTOSIS;
D O I：
10.1080/09273948.2019.1568502
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
Purpose: To determine the anti-inflammatory effects of dehydroxymethylepoxyquinomicin (DHMEQ), a nuclear factor-kappa B (NF-kappa B) inhibitor, on endotoxin-induced uveitis (EIU) in rats. Methods: EIU was induced by a subcutaneous injection of lipopolysaccharide (LPS) in Lewis rats. DHMEQ was injected intraperitoneally concurrently with the LPS. Aqueous humor was collected 24 h after the LPS injection. Isolated peritoneal exudate cells (PECs) were exposed to LPS with or without DHMEQ to determine the production of TNF-alpha, IL-6, and MCP-1. Results: DHMEQ significantly reduced the number of infiltrating cells, and the concentrations of proteins, TNF-alpha, and IL-6 in the aqueous humor. DHMEQ suppressed the production of TNF-alpha, IL-6, and MCP-1 from PECs. Immunochemistry revealed a reduction in the translocation of the NF-kappa B p65 into the nuclei in DHMEQ-exposed PECs. Conclusions: The results indicate that DHMEQ has anti-inflammatory effects on EIU and may be a promising agent to treat intraocular inflammation.
引用
收藏
页码：240 / 248
页数：9
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