Biochemical and molecular characterization of wheat genotypes determined by RAPD analysis

The Random Amplified Polymorphic DNA ( RAPD) technique was used to study the genetic diversity and relationships within the collection of the National Plant Genetics Resources of Wheat. A total of 413 fragments were amplified with 40 random decamer primers 46.97% of which were polymorphism. The aim of the work was to elaborate a dendrogram of genetic similarity between salt tolerant and salt sensitive and to divide collection into clusters. Forty primers were applied to 16 wheat cultivars different in the place of origin and 194 polymorphism bands were generated for each cultivar. The RAPD variation observed among these cultivars was low. The wheat genotypes were clustered in to one major group ( B) and two small groups ( A and C). The most salt sensitive distant genotypes in to the dendrogram were SW-20, which was genetically dissimilar with the other genotypes. It has been clearly shown that most of the cultivars except SW-20 possessed narrow genetic background. The information would be useful for future genome mapping programme as well as for the application of intellectual breeder right in the worldwide.