pH-Dependent Flavin Adenine Dinucleotide and Nicotinamide Adenine Dinucleotide Ultraviolet Resonance Raman (UVRR) Spectra at Intracellular Concentration

被引：3

作者：

Merk, Virginia ^{[1
,2
,3
]}

Speiser, Eugen ^{[3
]}

Werncke, Wolfgang ^{[3
]}

Esser, Norbert ^{[3
,4
]}

Kneipp, Janina ^{[1
,2
]}

机构：

[1] Humboldt Univ, Dept Chem, Berlin, Germany

[2] Humboldt Univ, Sch Analyt Sci Adlershof, Berlin, Germany

[3] ISAS Berlin, Berlin, Germany

[4] Tech Univ Berlin, Inst Solid State Phys, Dept Phys, Berlin, Germany

来源：

APPLIED SPECTROSCOPY | 2021年 / 75卷 / 08期

关键词：

Ultraviolet resonance Raman; UVRR; flavin adenine dinucleotide; FAD; nicotinamide adenine dinucleotide; NADH; adenine; pH dependence; protonation; NUCLEIC-ACID BASES; EXCITATION PROFILES; SPECTROSCOPY; MITOCHONDRIAL; NUCLEOTIDES; NADH; MONONUCLEOTIDE; CONFORMATION; DERIVATIVES;

D O I：

10.1177/00037028211025575

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

The ultraviolet resonance Raman spectra of the adenine-containing enzymatic redox cofactors nicotinamide adenine dinucleotide and flavin adenine dinucleotide in aqueous solution of physiological concentration are compared with the aim of distinguishing between them and their building block adenine in potential co-occurrence in biological materials. At an excitation wavelength of 266 nm, the spectra are dominated by the strong resonant contribution from adenine; nevertheless, bands assigned to vibrational modes of the nicotinamide and the flavin unit are found to appear at similar signal strength. Comparison of spectra measured at pH 7 with data obtained pH 10 and pH 3 shows characteristic changes when pH is increased or lowered, mainly due to deprotonation of the flavin and nicotinamide moieties, and protonation of the adenine, respectively.

引用

页码：994 / 1002

页数：9

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