Analysis of the Sorangicin Gene Cluster Reinforces the Utility of a Combined Phylogenetic/Retrobiosynthetic Analysis for Deciphering Natural Product Assembly by trans-AT PKS

被引：53

作者：

Irschik, Herbert ^{[1
]}

Kopp, Maren ^{[2
,3
]}

Weissman, Kira J. ^{[2
,3
]}

Buntin, Kathrin ^{[2
,3
]}

Piel, Joern ^{[4
]}

Mueller, Rolf ^{[1
,2
,3
]}

机构：

[1] Helmholtz Ctr Infect Res, D-38124 Braunschweig, Germany

[2] Univ Saarland, Helmholtz Ctr Infect Res, Helmholtz Inst Pharmaceut Res, D-66041 Saarbrucken, Germany

[3] Univ Saarland, Dept Pharmaceut Biotechnol, D-66041 Saarbrucken, Germany

[4] Univ Bonn, Kekule Inst Organ Chem & Biochem, D-53121 Bonn, Germany

来源：

CHEMBIOCHEM | 2010年 / 11卷 / 13期

关键词：

biosynthesis; phylogenetic analysis; polyketides; retrobiosynthesis; sorangicins; synthases; ACYL CARRIER PROTEIN; POLYKETIDE SYNTHASE; SUBSTRATE-SPECIFICITY; GLIDING BACTERIA; BIOSYNTHETIC MACHINERY; PEPTIDE SYNTHETASE; ACYLTRANSFERASE; ANTIBIOTICS; CELLULOSUM; SEQUENCE;

D O I：

10.1002/cbic.201000313

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(Chemical Equation Presented) A useful strategy: Deciphering biosynthesis by trans-AT polyketide synthases (PKS) is challenging due to their highly nonlinear organization. Our analysis of the trans-AT PKS responsible for sorangicin production in the myxobacterium Sorangium cellulosum So ce12 reinforces the utility of a combined phylogenetic/retrobiosynthetic approach for understanding this complex mode of biosynthesis. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：1840 / 1849

页数：10

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