Efficient porcine reproductive and respiratory syndrome virus entry in MARC-145 cells requires EGFR-PI3K-AKT-LIMK1-COFILIN signaling pathway

被引：15

作者：

Wang, Rui ^{[1
]}

Wang, Xin ^{[1
]}

Wu, Jia-qiang ^{[3
]}

Ni, Bo ^{[1
]}

Wen, Li-bing ^{[4
]}

Huang, Li ^{[1
]}

Liao, Ying ^{[2
]}

Tong, Guang-zhi ^{[2
]}

Ding, Chan ^{[2
]}

Mao, Xiang ^{[1
,2
]}

机构：

[1] Nanjing Agr Univ, Coll Vet Med, Nanjing, Jiangsu, Peoples R China

[2] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai, Peoples R China

[3] Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan, Peoples R China

[4] Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing, Jiangsu, Peoples R China

来源：

VIRUS RESEARCH | 2016年 / 225卷

关键词：

Porcine reproductive and respiratory; syndrome virus; Entry; Epidermal growth factor receptor; Cofilin; EPIDERMAL-GROWTH-FACTOR; AVIAN-SARCOMA VIRUS; FACTOR RECEPTOR; C-SRC; LIM-KINASE; HUMAN CYTOMEGALOVIRUS; ALVEOLAR MACROPHAGES; ACTIN DYNAMICS; PHOSPHORYLATION; ACTIVATION;

D O I：

10.1016/j.virusres.2016.09.005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Viruses have evolved diverse strategies to take over cellular machinery to facilitate their infection. In our studies presented here, we first demonstrated that Src kinase was involved in PRRSV entry in MARC-145 cells. Further studies demonstrated epidermal growth factor receptor (EGFR) was activated by the currently unknown mechanism(s) during PRRSV entry, which subsequently initiated EGFR downstream signal pathways, such as PI3K/AKT/LIMK1. Through these pathways, the virus entry signal was ultimately transferred to cofilin, which might regulate the actin fragmentation and reorganization to facilitate the virus penetration and cytoplasmic trafficking. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：23 / 32

页数：10

共 54 条

[11] MOLECULAR CHARACTERIZATION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS, A MEMBER OF THE ARTERIVIRUS GROUP [J].

CONZELMANN, KK ;

VISSER, N ;

VANWOENSEL, P ;

THIEL, HJ .

VIROLOGY, 1993, 193 (01) :329-339

[12] Virus-induced Abl and Fyn kinase signals permit coxsackievirus entry through epithelial tight junctions [J].

Coyne, CB ;

Bergelson, JM .

CELL, 2006, 124 (01) :119-131

[13] Porcine Arterivirus infection of alveolar macrophages is mediated by sialic acid on the virus [J].

Delputte, PL ;

Nauwynck, HJ .

JOURNAL OF VIROLOGY, 2004, 78 (15) :8094-8101

[14] The Epidermal Growth Factor Receptor (EGFR) Promotes Uptake of Influenza A Viruses (IAV) into Host Cells [J].

Eierhoff, Thorsten ;

Hrincius, Eike R. ;

Rescher, Ursula ;

Ludwig, Stephan ;

Ehrhardt, Christina .

PLOS PATHOGENS, 2010, 6 (09)

[15] The PI3K/Akt pathway is involved in early infection of some exogenous avian leukosis viruses [J].

Feng, Shao-zhen ;

Cao, Wei-sheng ;

Liao, Ming .

JOURNAL OF GENERAL VIROLOGY, 2011, 92 :1688-1697

[16] Discovery of a small arterivirus gene that overlaps the GP5 coding sequence and is important for virus production [J].

Firth, Andrew E. ;

Zevenhoven-Dobbe, Jessika C. ;

Wills, Norma M. ;

Go, Yun Young ;

Balasuriya, Udeni B. R. ;

Atkins, John F. ;

Snijder, Eric J. ;

Posthuma, Clara C. .

JOURNAL OF GENERAL VIROLOGY, 2011, 92 :1097-1106

[17] Signalling through class I PI3Ks in mammalian cells [J].

Hawkins, P. T. ;

Anderson, K. E. ;

Davidson, K. ;

Stephens, L. R. .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2006, 34 :647-662

[18] Integrin β1 Mediates Vaccinia Virus Entry through Activation of PI3K/Akt Signaling [J].

Izmailyan, Roza ;

Hsao, Jye-Chian ;

Chung, Che-Sheng ;

Chen, Chein-Hung ;

Hsu, Paul Wei-Che ;

Liao, Chung-Lin ;

Chang, Wen .

JOURNAL OF VIROLOGY, 2012, 86 (12) :6677-6687

[19] Novel structural protein in porcine reproductive and respiratory syndrome virus encoded by an alternative ORF5 present in all arteriviruses [J].

Johnson, Craig R. ;

Griggs, Theodor F. ;

Gnanandarajah, Josephine ;

Murtaugh, Michael P. .

JOURNAL OF GENERAL VIROLOGY, 2011, 92 :1107-1116

[20] Porcine reproductive and respiratory syndrome virus enters cells through a low pH-dependent endocytic pathway [J].

Kreutz, LC ;

Ackermann, MR .

VIRUS RESEARCH, 1996, 42 (1-2) :137-147

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