Efficient porcine reproductive and respiratory syndrome virus entry in MARC-145 cells requires EGFR-PI3K-AKT-LIMK1-COFILIN signaling pathway

被引：15

作者：

Wang, Rui ^{[1
]}

Wang, Xin ^{[1
]}

Wu, Jia-qiang ^{[3
]}

Ni, Bo ^{[1
]}

Wen, Li-bing ^{[4
]}

Huang, Li ^{[1
]}

Liao, Ying ^{[2
]}

Tong, Guang-zhi ^{[2
]}

Ding, Chan ^{[2
]}

Mao, Xiang ^{[1
,2
]}

机构：

[1] Nanjing Agr Univ, Coll Vet Med, Nanjing, Jiangsu, Peoples R China

[2] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai, Peoples R China

[3] Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan, Peoples R China

[4] Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing, Jiangsu, Peoples R China

来源：

VIRUS RESEARCH | 2016年 / 225卷

关键词：

Porcine reproductive and respiratory; syndrome virus; Entry; Epidermal growth factor receptor; Cofilin; EPIDERMAL-GROWTH-FACTOR; AVIAN-SARCOMA VIRUS; FACTOR RECEPTOR; C-SRC; LIM-KINASE; HUMAN CYTOMEGALOVIRUS; ALVEOLAR MACROPHAGES; ACTIN DYNAMICS; PHOSPHORYLATION; ACTIVATION;

D O I：

10.1016/j.virusres.2016.09.005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Viruses have evolved diverse strategies to take over cellular machinery to facilitate their infection. In our studies presented here, we first demonstrated that Src kinase was involved in PRRSV entry in MARC-145 cells. Further studies demonstrated epidermal growth factor receptor (EGFR) was activated by the currently unknown mechanism(s) during PRRSV entry, which subsequently initiated EGFR downstream signal pathways, such as PI3K/AKT/LIMK1. Through these pathways, the virus entry signal was ultimately transferred to cofilin, which might regulate the actin fragmentation and reorganization to facilitate the virus penetration and cytoplasmic trafficking. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：23 / 32

页数：10

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