CSK promotes innate immune response to DNA virus by phosphorylating MITA

被引：14

作者：

Gao, Peng ^{[1
]}

Hu, Ming-Ming ^{[1
]}

Shu, Hong-Bing ^{[1
]}

机构：

[1] Wuhan Univ, Zhongnan Hosp, Med Res Inst, Dept Infect Dis, Wuhan 430071, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2020年 / 526卷 / 01期

基金：

中国国家自然科学基金;

关键词：

DNA virus; MITA; CSK; Tyrosine phosphorylation; Innate immune response; TYROSINE KINASES; RECOGNITION; SENSOR; COMPLEX; GENE; CGAS;

D O I：

10.1016/j.bbrc.2020.03.069

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Upon detection of viral DNA, the cytoplasmic DNA sensor cyclic GMP-AMP (cGAMP) synthase (cGAS) utilizes GTP and ATP as substrates to synthesize the second messenger molecule 2'3'cyclic GMP-AMP (cGAMP), which binds to the ER-associated adaptor protein MITA/STING to signal innate antiviral response to DNA virus. How the cGAS-MITA pathways are post-translationally regulated is not fully understood. In this study, we identified the tyrosine kinase CSK as a positive regulator of cGAS-MITA mediated innate antiviral response. CSK-deficiency inhibits DNA virus-triggered induction of downstream antiviral effector genes. Following DNA virus infection, CSK phosphorylates MITA at Y240 and Y245, which is important for its activation. These results suggest that CSK plays a role in modulating innate immune response to DNA virus. (C) 2020 Elsevier Inc. All rights reserved.

引用

页码：199 / 205

页数：7

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