Evaluation of the tube method for concentrating feline platelets: cellular study

被引：8

作者：

Silva, R. F. ^{[1
,2
]}

Rezende, C. M. F. ^{[2
]}

Paes-Leme, F. O. ^{[2
]}

Carmona, J. U. ^{[1
]}

机构：

[1] Univ Caldas, Dept Salud Anim, Grp Invest Terapia Regenerat, Manizales, Caldas, Colombia

[2] Univ Fed Minas Gerais, Escola Vet, Belo Horizonte, MG, Brazil

来源：

ARCHIVOS DE MEDICINA VETERINARIA | 2011年 / 43卷 / 02期

关键词：

cat; autologous platelet rich plasma; regenerative medicine; tube method; RICH PLASMA; AGGREGATION;

D O I：

10.4067/S0301-732X2011000200013

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The aim of this study was to evaluate a manual tube method for concentrating cat platelets and consequently produce autologous platelet concentrates (APCs) or platelet rich plasma (PRP), for clinical and experimental purposes. The PRP were obtained by venous blood collection in tubes with ACD solution that were spun at 85 g for 6 minutes. The cell counts of whole blood and PRP, were statistically different (P < 0.01) between cell values for platelets, leukocytes, granulocytes, monocytes, but not for lymphocytes. We found a strong positive correlation (p = 0,734 P = 0,01) between the number of platelets in whole blood and platelets in the PRP. The collection efficiency of platelets was 50% and the concentration of platelets was 147.7% higher in PRP in comparison with the whole blood samples. The results indicated that this simple centrifugation tube method allows concentrating cat platelets.

引用

页码：187 / 190

页数：4

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