Low genetic connectivity in an estuarine fish with pelagic larvae

We evaluated the spatial scale of metapopulation structure and genetic connectivity in rainbow smelt, Osimerus mordax, using eight microsatellite loci at 22 spawning locations throughout Newfoundland and Labrador. Consistent with low gene flow and limited dispersal, significant genetic structuring (F-ST approximate to 0.11) was present at small spatial scales (< 200 km). Moreover, strong isolation by distance (IBD, P < 0.001, r(2) = 0.47) was observed, which was linear at small scales and nonlinear at large distances (> 200 km). We hypothesized that despite high dispersal potential associated with a pelagic larval stage, behaviours restricting gene flow may result in structuring at the estuary scale. Multidimensional scaling and neighbour-joining of multilocus genotypes indicate some bay-scale associations. However, a comparison of FST values and IBD residuals at both estuary and bay scales indicated low Structure within and elevated structure among estuaries. Estuarine Structuring was further supported by the presence of significant small-scale IBD within several coastal embayments (50-100 km), as well as Bayesian clustering consistent with estuarine-scale independence. Finally, estimates of dispersal based on the IBD relationship are consistent with local estuarine recruitment (< 1.5 km.generation(-1)). We conclude that the unexpectedly high genetic structure observed is consistent with behavioral influences reducing dispersal, supporting previous work implicating active larval retention.