Capillary Electrophoresis Involving in High Efficiency Screening for Aptamers

Systematic evolution of ligands by exponential enrichment (SELEX) is a universal technology for aptamers. screening. Accurate and efficient screening methods and strategy design are the key to successful screening of aptamers. This article briefly summarizes our works of capillary electrophoresis involving in the efficient screening of aptamers since 2007. The review also introduces the employment of capillary electrophoresis in pre-screening, screening and post-screening SELEX process, classification and screening strategies against different protein targets, multiple capillary electrophoresis screening modes, and aptamers screening for multi-scale targets. Finally, combined with the current research progress of aptamer screening in the world, some remaining issues are proposed.