Magnetite/CdTe magnetic-fluorescent composite nanosystem for magnetic separation and bio-imaging

被引：27

作者：

Kale, Anup ^{[2
]}

Kale, Sonia ^{[1
]}

Yadav, Prasad ^{[2
]}

Gholap, Haribhau ^{[2
]}

Pasricha, Renu ^{[3
]}

Jog, J. P. ^{[2
]}

Lefez, Benoit ^{[4
]}

Hannoyer, Beatrice ^{[4
]}

Shastry, Padma ^{[1
]}

Ogale, Satishchandra ^{[2
]}

机构：

[1] Natl Ctr Cell Sci, Pune 411007, Maharashtra, India

[2] CSIR, Natl Chem Lab, Pune 411008, Maharashtra, India

[3] CSIR, Natl Phys Lab, New Delhi 110012, India

[4] Univ Rouen, GPM UMR CNRS 6634, F-76801 Etienne Du Rouvray, France

来源：

NANOTECHNOLOGY | 2011年 / 22卷 / 22期

关键词：

SMALL-ANGLE NEUTRON; ONE-POT SYNTHESIS; QUANTUM DOTS; NANOPARTICLES; NANOCOMPOSITES; FACILE; FUNCTIONALIZATION; INTERFACE; CRYSTALS; SURFACE;

D O I：

10.1088/0957-4484/22/22/225101

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

A new synthesis protocol is described to obtain a CdTe decorated magnetite bifunctional nanosystem via dodecylamine (DDA) as cross linker. High resolution transmission electron microscopy (HRTEM), energy-dispersive x-ray spectroscopy (EDAX), vibrating sample magnetometry (VSM), Fourier transform infrared spectroscopy (FTIR), diffuse reflectance spectroscopy (DRS) and fluorescence microscopy are used to characterize the constitution, size, composition and physical properties of these superparamagnetic-fluorescent nanoparticles. These CdTe decorated magnetite nanoparticles were then functionalized with anti-epidermal growth factor receptor (EGFR) antibody to specifically target cells expressing this receptor. The EGFR is a transmembrane glycoprotein and is expressed on tumor cells from different tissue origins including human leukemic cell line Molt-4 cells. The magnetite-CdTe composite nanosystem is shown to perform excellently for specific selection, magnetic separation and fluorescent detection of EGFR positive Molt-4 cells from a mixed population. Flow cytometry and confocal laser scanning microscopy results show that this composite nanosystem has great potential in antibody functionalized magnetic separation and imaging of cells using cell surface receptor antibody.

引用

页数：12

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[1] Luminescence and stability of aqueous thioalkyl acid capped CdSe/ZnS quantum dots correlated to ligand ionization [J].