Engineering of multiple modular pathways for high-yield production of 5-aminolevulinic acid in Escherichia coli

被引：56
作者：
Zhang, Junli [1 ,2 ,3 ]
Weng, Huanjiao [2 ,3 ]
Zhou, Zhengxiong [2 ,3 ]
Du, Guocheng [2 ,3 ]
Kang, Zhen [2 ,3 ]
机构：
[1] Taishan Med Univ, Sch Life Sci, Tai An 271016, Shandong, Peoples R China
[2] Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China
[3] Jiangnan Univ, Key Lab Carbohydrate Chem & Biotechnol, Minist Educ, Wuxi 214122, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2019年 / 274卷
关键词：
5-aminolevulinic acid; ALA dehydratase; Pyridoxal 5 '-phosphate; Metabolic engineering; Escherichia coli; STATIONARY-PHASE PROMOTERS; DELTA-AMINOLEVULINIC-ACID; CORYNEBACTERIUM-GLUTAMICUM; EFFICIENT PRODUCTION; PLASMID STABILITY; BIOSYNTHESIS; OPTIMIZATION; COPRODUCTION; EXPRESSION; MOLECULES;
D O I：
10.1016/j.biortech.2018.12.004
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
5-aminolevulinic acid (ALA), an important precursor of tetrapyrroles, has various applications in medicine and agriculture fields. Several methods have been adopted to enhance ALA synthesis in our previous studies. In this study, systematic metabolic engineering strategies were implemented to further improve ALA production in Escherichia coli. Firstly, hemA and hemL with different strength of RBS from the artificially constructed mutation libraries were randomly assembled to balance metabolic flux. Then the expression of ALA dehydratase was rationally regulated by replacing promoter with fliCp to weaken ALA catabolism. Besides, the activity of glutamate-1-semialdehyde aminotransferase was increased through strengthening the native biosynthesis pathway of cofactor pyridoxal 5'-phosphate. Moreover, plasmid stability was improved by 21.4% by deleting recA and endA in the recombinant. Finally, stepwise improvements in ALA production were increased to 5.25 g/L with a pH two-stage strategy in a 3-L fermenter. This study proved the importance of metabolic balance in the pathway.
引用
收藏
页码：353 / 360
页数：8
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