Chemical fingerprinting of Gardenia jasminoides Ellis by HPLC-DAD-ESI-MS combined with chemometrics methods

被引：69

作者：

Han, Yan ^{[1
,2
,3
]}

Wen, Jun ^{[1
,2
]}

Zhou, Tingting ^{[1
,2
]}

Fan, Guorong ^{[1
,2
]}

机构：

[1] Second Mil Med Univ, Sch Pharm, Shanghai 200433, Peoples R China

[2] Second Mil Med Univ, Shanghai Key Lab Pharmaceut Metabolite Res, Sch Pharm, Shanghai 200433, Peoples R China

[3] Fujian Univ Tradit Chinese Med, Coll Pharm, Fuzhou 350108, Peoples R China

来源：

FOOD CHEMISTRY | 2015年 / 188卷

关键词：

Gardenia jasminoides Ellis; Similarity evaluation; Principal components analysis (PCA); Hierarchical clustering analysis (HCA); HPLC-DAD-ESI-MS; Quality evaluation; TANDEM MASS-SPECTROMETRY; FRUIT EXTRACTS; IDENTIFICATION; QUANTIFICATION; CONSTITUENTS; GLYCOSIDES; FRUCTUS;

D O I：

10.1016/j.foodchem.2015.05.039

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A fingerprint analysis method has been developed for characterization and discrimination of Gardenia jasminoides Ellis from different areas. The chemometrics methods including similarity evaluation, principal components analysis (PCA) and hierarchical clustering analysis (HCA) were introduced to identify more useful chemical markers for improving the quality control standard of dried ripe fruits of G. jasminoides Ellis. Then the selected chemical markers were analyzed by high performance liquid chromatography diode array detection-electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS) qualitatively and quantitatively. 23 characteristic peaks were assigned while 19 peaks of them were identified by comparing retention times, UV and MS spectra with authentic compounds or literature data. Moreover, 14 of them were determined quantitatively which could effectively evaluate the quality of G. jasminoides Ellis. This study was expected to provide comprehensive information for the quality evaluation of G. jasminoides Ellis, which would be a valuable reference for further study and development of this herb and related medicinal products. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：648 / 657

页数：10

共 21 条

[1] LC-MSn identification and characterization of the phenolic compounds from the fruits of Flacourtia indica (Burm. F.) Merr. and Flacourtia inermis Roxb. [J].