Analysis of free glycerol in biodiesel using an electrochemical assay based on a two-enzyme platinum microelectrode system

被引：13

作者：

Pegas, Michelle Macedo ^{[1
]}

Amado, Roberto Salgado ^{[1
]}

de Castro, Eduardo Vieira ^{[1
]}

D'Elia, Eliane ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Quim, Ctr Tecnol, Ave Athos da Silveira Ramos 149,Bloco A,Lab 634A, BR-21941909 Rio de Janeiro, Brazil

来源：

JOURNAL OF APPLIED ELECTROCHEMISTRY | 2010年 / 40卷 / 11期

关键词：

Microelectrodes; Free glycerol; Hydrogen peroxide; Biodiesel amperometric detection; AMPEROMETRIC DETERMINATION; VEGETABLE-OILS; BIOSENSORS;

D O I：

10.1007/s10800-010-0168-9

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

An electroenzymatic method based on two coupled enzymatic activities (glycerokinase and glycerol-3-phosphate oxidase) was developed using a platinum microelectrode for the determination of free glycerol in biodiesel samples. The electroenzymatic method proposed showed a good linear correlation coefficient (R = 0.9894), with a linear response in the concentration range of 8.2 x 10(-5) to 5.7 x 10(-4)% (w/w) and a limit of detection of 6.0 x 10(-5)% (w/w). The results obtained for the free glycerol content of select biodiesel samples were compared with their gas chromatography (GC) analyses. The relative errors for glycerol determination using this enzymatic-amperometric method were in the range of -8.0 to 3.0%. The proposed method was shown to be promising for the analysis of glycerol in biodiesel samples and a simple and inexpensive method in comparison to gas chromatography.

引用

页码：2061 / 2063

页数：3