Different Expressions and DNA Methylation Patterns of Lysophosphatidic Acid Receptor Genes in Mouse Tumor Cells

被引：12

作者：

Okabe, Kyoko ^{[1
]}

Hayashi, Mai ^{[1
]}

Wakabayashi, Naoko ^{[1
]}

Yamawaki, Yasuna ^{[1
]}

Teranishi, Miki ^{[1
]}

Fukushima, Nobuyuki ^{[2
]}

Tsujiuchi, Toshifumi ^{[1
]}

机构：

[1] Kinki Univ, Fac Sci & Technol, Dept Life Sci, Div Canc Biol & Bioinformat, Higashiosaka, Osaka 577, Japan

[2] Kinki Univ, Fac Sci & Engn, Dept Life Sci, Div Mol Neurobiol, Osaka 5778502, Japan

来源：

PATHOBIOLOGY | 2010年 / 77卷 / 06期

关键词：

Mouse; Lysophosphatidic acid; Lysophosphatidic acid receptor; DNA methylation; Cancer cell; PROTEIN-COUPLED RECEPTOR; OVARIAN-CANCER; LPA; IMPLANTATION; GROWTH; HAIR; MICE;

D O I：

10.1159/000321898

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Objective: Lysophosphatidic acid (LPA) receptors act as several biological effectors through LPA, which is a bioactive phospholipid. Recently, aberrant expressions of LPA receptor genes due to DNA methylation have been detected in several tumor cells. In this study, we measured expression levels and DNA methylation status of LPA receptor genes in mouse tumor cells, LL/2 lung carcinoma, B16F0 melanoma, FM3A mammary carcinoma and L1210 leukemia cells, compared with normal tissues. Methods: Total RNAs were extracted and RT-PCR analysis was performed. For DNA methylation status, bisulfite sequencing analysis was carried out, comparing outcomes with other tumor cells and normal tissues. Results: The expressions of LPA1 gene were shown in LL/2, but not in B16F0, FM3A and L1210 cells. While the LPA2 gene was expressed in all 4 tumor cells, the LPA3 gene was unexpressed in them. The LPA1 and LPA3 unexpressed cells were highly methylated, although normal tissues were all unmethylated. The DNA methylation status was correlated with gene expression levels in cancer cells. Conclusion: The present results demonstrate that DNA methylation patterns of LPA receptor genes are dependent on cancer cell types, suggesting that LPA receptors may be new molecular targets for therapeutic approaches and chemoprevention. Copyright (C) 2011 S. Karger AG, Basel

引用

页码：309 / 314

页数：6

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